ArticleBMC evolutionary biology2004
The iron-sulfur cluster assembly genes iscS and iscU of Entamoeba histolytica were acquired by horizontal gene transfer.
Article in BMC evolutionary biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 66 citations in OpenAlex.
- Structural heterogeneity of iron-sulfur cluster scaffold protein IscU: Metamorphic or partially folded?Structural dynamics (Melville, N.Y.) · 2026Review
- Encystation and Stress Responses under the Control of Ubiquitin-like Proteins in Pathogenic Amoebae.Microorganisms · 2023Review
- On the Origin of Iron/Sulfur Cluster Biosynthesis in Eukaryotes.Frontiers in microbiology · 2019Article
- Fe-S Cluster Assembly in Oxymonads and Related Protists.Molecular biology and evolution · 2018Article
- Localization of Fe-S Biosynthesis Machinery in Cryptosporidium parvum Mitosome.The Journal of eukaryotic microbiology · 2018Article
- Novel Hydrogenosomes in the Microaerophilic Jakobid Stygiella incarcerata.Molecular biology and evolution · 2016Article
- Diversity and origins of anaerobic metabolism in mitochondria and related organelles.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- Turning Saccharomyces cerevisiae into a Frataxin-Independent Organism.PLoS genetics · 2015Article
- Lateral gene transfer and gene duplication played a key role in the evolution of Mastigamoeba balamuthi hydrogenosomes.Molecular biology and evolution · 2015Article
- A novel Mitosomal β-barrel Outer Membrane Protein in Entamoeba.Scientific reports · 2015Article
- Identification of a novel pentatricopeptide repeat subfamily with a C-terminal domain of bacterial origin acquired via ancient horizontal gene transfer.BMC research notes · 2013Article
- Divergence of Erv1-associated mitochondrial import and export pathways in trypanosomes and anaerobic protists.Eukaryotic cell · 2013Article
- Parasites FeS up: iron-sulfur cluster biogenesis in eukaryotic pathogens.PLoS pathogens · 2013Article
- Entamoeba histolytica: a snapshot of current research and methods for genetic analysis.Current opinion in microbiology · 2012Review
- Algal genes in aplastidic eukaryotes are not necessarily derived from historical plastids.Mobile genetic elements · 2012Article
- The Trichomonas vaginalis hydrogenosome proteome is highly reduced relative to mitochondria, yet complex compared with mitosomes.International journal for parasitology · 2011Article
- Mitosomes in trophozoites and cysts of the reptilian parasite Entamoeba invadens.Eukaryotic cell · 2011Article
- The essentials of protein import in the degenerate mitochondrion of Entamoeba histolytica.PLoS pathogens · 2010Article
- Diversity and reductive evolution of mitochondria among microbial eukaryotes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2010Review
- Human amebiasis: breaking the paradigm?International journal of environmental research and public health · 2010Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIron-sulfur (FeS) proteins are present in all living organisms and play important roles in electron transport and metalloenzyme catalysis. The maturation of FeS proteins in eukaryotes is an essential function of mitochondria, but little is known about this process in amitochondriate eukaryotes. Here we report on the identification and analysis of two genes encoding critical FeS cluster (Isc) biosynthetic proteins from the amitochondriate human pathogen Entamoeba histolytica.
resultsE. histolytica IscU and IscS were found to contain all features considered essential for their biological activity, including amino acid residues involved in substrate and/or co-factor binding. The IscU protein differs significantly from other eukaryotic homologs and resembles the long type isoforms encountered in some bacteria. Phylogenetic analyses of E. histolytica IscS and IscU showed a close relationship with homologs from Helicobacter pylori and Campylobacter jejuni, to the exclusion of mitochondrial isoforms.
conclusionsThe bacterial-type FeS cluster assembly genes of E. histolytica suggest their lateral acquisition from epsilon proteobacteria. This is a clear example of horizontal gene transfer (HGT) from eubacteria to unicellular eukaryotic organisms, a phenomenon known to contribute significantly to the evolution of eukaryotic genomes.
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