ArticleInfection and immunity2004
Molecular cloning and characterization of cgt, the Brucella abortus cyclic beta-1,2-glucan transporter gene, and its role in virulence.
Article in Infection and immunity, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Quantitative proteomics identifies GntR as a novel potential DIVA antigen for bovine brucellosis.Applied microbiology and biotechnology · 2026Article
- A β-1,2-glucan-associated glycoside hydrolase family 1 β-glucosidase from Streptomyces griseus.Protein science : a publication of the Protein Society · 2025Article
- Discovery of Anomer-Inverting Transglycosylase: Cyclic Glucohexadecaose-Producing Enzyme fromJournal of the American Chemical Society · 2024Article
- Development of a scalable recombinant system for cyclic beta-1,2-glucans production.Microbial cell factories · 2024Article
- Immunosuppressive Mechanisms in Brucellosis in Light of Chronic Bacterial Diseases.Microorganisms · 2022Review
- Global gene-expression profiles of intracellular survival of the BruAb2_1031 gene mutated Brucella abortus in professional phagocytes, RAW 264.7 cells.BMC microbiology · 2018Article
- Iron-dependent reconfiguration of the proteome underlies the intracellular lifestyle of Brucella abortus.Scientific reports · 2017Article
- Osmoregulated Periplasmic Glucans.EcoSal Plus · 2017Article
- New insights into the biological role of the osmoregulated periplasmic glucans in pathogenic and symbiotic bacteria.Environmental microbiology reports · 2015Review
- Interaction network and localization of Brucella abortus membrane proteins involved in the synthesis, transport, and succinylation of cyclic β-1,2-glucans.Journal of bacteriology · 2015Article
- RNA-Seq analysis of the multipartite genome of Rhizobium etli CE3 shows different replicon contributions under heat and saline shock.BMC genomics · 2014Article
- Brucella cyclic β-1,2-glucan plays a critical role in the induction of splenomegaly in mice.PloS one · 2014Article
- The Aggregation of Brucella abortus Occurs Under Microaerobic Conditions and Promotes Desiccation Tolerance and Biofilm Formation.The open microbiology journal · 2013Article
- Host-Brucella interactions and the Brucella genome as tools for subunit antigen discovery and immunization against brucellosis.Frontiers in cellular and infection microbiology · 2013Review
- Identification of cold-temperature-regulated genes in Flavobacterium psychrophilum.Applied and environmental microbiology · 2011Article
- Importance of Lipopolysaccharide and Cyclic β-1,2-Glucans in Brucella-Mammalian Infections.International journal of microbiology · 2010Article
- Study of free oligosaccharides derived from the bacterial N-glycosylation pathway.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Functional mapping of Brucella abortus cyclic beta-1,2-glucan synthase: identification of the protein domain required for cyclization.Journal of bacteriology · 2009Article
- ATP-Binding Cassette Systems of Brucella.Comparative and functional genomics · 2009Article
- A glycosyltransferase with a length-controlling activity as a mechanism to regulate the size of polysaccharides.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
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Authors and funding
4 authors.
Funding
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Abstract
The animal pathogen Brucella abortus contains a gene cgt, which complemented Sinorhizobium meliloti nodule development (ndvA) and Agrobacterium tumefaciens chromosomal virulence (chvA) mutants. Complemented strains recovered the presence of anionic cyclic beta-1,2-glucan, motility, tumor induction in A. tumefaciens, and nodule occupancy in S. meliloti, all traits strictly associated with the presence of cyclic beta-1,2-glucan in the periplasm. Nucleotide sequencing revealed that B. abortus cgt contains a 1,797-bp open reading frame coding for a predicted membrane protein of 599 amino acids (65.9 kDa) that is 58.5 and 59.9% identical to S. meliloti NdvA and A. tumefaciens ChvA, respectively. Additionally, B. abortus cgt, like S. meliloti ndvA and A. tumefaciens chvA possesses ATP-binding motifs and the ABC signature domain features of a typical ABC transporter. Characterization of Cgt was carried out by the construction of null mutants in B. abortus 2308 and S19 backgrounds. Both mutants do not transport cyclic beta-1,2-glucan to the periplasm, as shown by the absence of anionic cyclic glucan, and they display reduced virulence in mice and defective intracellular multiplication in HeLa cells. These results suggest that cyclic beta-1,2-glucan must be transported into the periplasmatic space to exert its action as a virulence factor.
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