ArticleInfection and immunity2004
Bovine ileal intraepithelial lymphocytes represent target cells for Shiga toxin 1 from Escherichia coli.
Article in Infection and immunity, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Effects of Maternal Supplementation with Organic Trace Minerals including Zinc, Manganese, Copper, and Cobalt during the Late and Post-Partum Periods on the Health and Immune Status of Japanese Black Calves.Animals : an open access journal from MDPI · 2023Article
- Characterization of Bovine Intraepithelial T Lymphocytes in the Gut.Pathogens (Basel, Switzerland) · 2023Article
- The Role ofToxins · 2020Review
- Molecular Biology ofToxins · 2020Review
- Direct Manipulation of T Lymphocytes by Proteins of Gastrointestinal Bacterial Pathogens.Infection and immunity · 2018Review
- Host mechanisms involved in cattle Escherichia coli O157 shedding: a fundamental understanding for reducing foodborne pathogen in food animal production.Scientific reports · 2017Article
- Immune Response in Calves Vaccinated with Type Three Secretion System Antigens and Shiga Toxin 2B Subunit of Escherichia coli O157:H7.PloS one · 2017Article
- A Topographical Atlas of Shiga Toxin 2e Receptor Distribution in the Tissues of Weaned Piglets.Toxins · 2016Article
- Evaluation of biological safety in vitro and immunogenicity in vivo of recombinant Escherichia coli Shiga toxoids as candidate vaccines in cattle.Veterinary research · 2015Article
- Strain-dependent cellular immune responses in cattle following Escherichia coli O157:H7 colonization.Infection and immunity · 2014Article
- Inflammatory effects of Edwardsiella ictaluri lipopolysaccharide modifications in catfish gut.Infection and immunity · 2014Article
- Characterization of Shiga toxin - producing Escherichia coli infections in beef feeder calves and the effectiveness of a prebiotic in alleviating Shiga toxin - producing Escherichia coli infections.Irish veterinary journal · 2013Article
- Attaching-effacing Escherichia coli infections in cattle.The Veterinary clinics of North America. Food animal practice · 2010Article
- Maternally and naturally acquired antibodies to Shiga toxins in a cohort of calves shedding Shiga-toxigenic Escherichia coli.Applied and environmental microbiology · 2009Article
- Grazing protozoa and the evolution of the Escherichia coli O157:H7 Shiga toxin-encoding prophage.Proceedings. Biological sciences · 2007Article
- Shiga toxin and Shiga toxin-encoding phage do not facilitate Escherichia coli O157:H7 colonization in sheep.Applied and environmental microbiology · 2007Article
- Bovine immune response to shiga-toxigenic Escherichia coli O157:H7.Clinical and vaccine immunology : CVI · 2006Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The discovery that bovine peripheral lymphocytes are sensitive to Stx1 identified a possible mechanism for the persistence of infections with Shiga toxin (Stx)-producing Escherichia coli (STEC) in the bovine reservoir host. If intraepithelial lymphocytes (IEL) are also sensitive to Stx1, the idea that Stx1 affects inflammation in the bovine intestine is highly attractive. To prove this hypothesis, ileal IEL (iIEL) were prepared from adult cattle, characterized by flow cytometry, and subjected to functional assays in the presence and absence of purified Stx1. We found that 14.9% of all iIEL expressed Gb(3)/CD77, the Stx1 receptor on bovine lymphocytes, and 7.9% were able to bind the recombinant B subunit of Stx1. The majority of Gb(3)/CD77(+) cells were activated CD3(+) CD6(+) CD8 alpha(+) T cells, whereas only some CD4(+) T cells and B cells expressed Gb(3)/CD77. However, Stx1 blocked the mitogen-induced transformation to enlarged blast cells within all subpopulations to a similar extent and significantly reduced the percentage of Gb(3)/CD77(+) cells. Although Stx1 did not affect the natural killer cell activity of iIEL, the toxin accelerated the synthesis of interleukin-4 (IL-4) mRNA and reduced the amount of IL-8 mRNA in bovine iIEL cultures. Because the intestinal system comprises a rich network of interactions between different types of cells and any dysfunction may influence the course of intestinal infections, this demonstration that Stx1 can target bovine IEL may be highly relevant for our understanding of the interplay between STEC and its reservoir host.
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