ArticleRNA (New York, N.Y.)2004
In vivo recruitment of exon junction complex proteins to transcription sites in mammalian cell nuclei.
Article in RNA (New York, N.Y.), 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
44 citing papers in PubMed, 85 citations in OpenAlex.
- A unified photosensitizer platform forbioRxiv : the preprint server for biology · 2026Article
- RNA export through the nuclear pore complex is directional.Nature communications · 2022Article
- Sending the message: specialized RNA export mechanisms in trypanosomes.Trends in parasitology · 2022Review
- Imaging within single NPCs reveals NXF1's role in mRNA export on the cytoplasmic side of the pore.The Journal of cell biology · 2019Article
- Depletion of the MFAP1/SPP381 Splicing Factor Causes R-Loop-Independent Genome Instability.Cell reports · 2019Article
- Article
- RNA biology of disease-associated microsatellite repeat expansions.Acta neuropathologica communications · 2017Review
- Integration of mRNP formation and export.Cellular and molecular life sciences : CMLS · 2017Review
- PKR activation and eIF2α phosphorylation mediate human globin mRNA splicing at spliceosome assembly.Cell research · 2017Article
- UAP56 is a conserved crucial component of a divergent mRNA export pathway in Toxoplasma gondii.Molecular microbiology · 2016Article
- A Conserved Nuclear Cyclophilin Is Required for Both RNA Polymerase II Elongation and Co-transcriptional Splicing in Caenorhabditis elegans.PLoS genetics · 2016Article
- The exon junction complex as a node of post-transcriptional networks.Nature reviews. Molecular cell biology · 2016Review
- Intranuclear binding in space and time of exon junction complex and NXF1 to premRNPs/mRNPs in vivo.The Journal of cell biology · 2015Article
- Multiple Export Mechanisms for mRNAs.Cells · 2015Review
- Review
- Aly and THO are required for assembly of the human TREX complex and association of TREX components with the spliced mRNA.Nucleic acids research · 2013Article
- A genome-wide siRNA screen to identify host factors necessary for growth of the parasite Toxoplasma gondii.PloS one · 2013Article
- Human CWC22 escorts the helicase eIF4AIII to spliceosomes and promotes exon junction complex assembly.Nature structural & molecular biology · 2012Article
- Perispeckles are major assembly sites for the exon junction core complex.Molecular biology of the cell · 2012Article
- Spliceosome assembly is coupled to RNA polymerase II dynamics at the 3' end of human genes.Nature structural & molecular biology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Studies over the past years indicate that there is extensive coupling between nuclear export of mRNA and pre-mRNA processing. Here, we visualized the distribution of exon junction complex (EJC) proteins and RNA export factors relative to sites of abundant pre-mRNA synthesis in the nucleus. We analyzed both HeLa cells infected with adenovirus and murine erythroleukemia (MEL) cells stably transfected with the human beta-globin gene. Using in situ hybridization and confocal microscopy, we observe accumulation of EJC proteins (REF/Aly, Y14, SRm160, UAP56, RNPS1, and Magoh) and core spliceosome components (U snRNPs) at sites of transcription. This suggests that EJC proteins bind stably to pre-mRNA cotranscriptionally. No concentration of the export factors NXF1/TAP, p15, and Dbp5 was detected on nascent transcripts, arguing that in mammalian cells these proteins bind the mRNA shortly before or after release from the sites of transcription. These results also suggest that binding of EJC proteins to the mRNA is not sufficient to recruit TAP-p15, consistent with recent findings showing that the EJC does not play a crucial role in mRNA export. Contrasting to the results obtained in MEL cells expressing normal human beta-globin transcripts, mutant pre-mRNAs defective in splicing and 3'end processing do not colocalize with SRm160, REF, UAP56, or Sm proteins. This shows that the accumulation of EJC proteins at transcription sites requires efficient processing of the nascent pre-mRNAs, arguing that transcription per se is not sufficient for the stable assembly of the EJC.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.