ArticleRNA (New York, N.Y.)2004
Mitochondrial 3' tRNA editing in the jakobid Seculamonas ecuadoriensis: a novel mechanism and implications for tRNA processing.
Article in RNA (New York, N.Y.), 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 38 citations in OpenAlex.
- Mitochondrial genome sequence of the protistFrontiers in microbiology · 2023Article
- Hopeful monsters: unintended sequencing of famously malformed mite mitochondrial tRNAs reveals widespread expression and processing of sense-antisense pairs.NAR genomics and bioinformatics · 2021Article
- The mitochondrial genomes of two walnut pests,PeerJ · 2018Article
- Repairing tRNA termini: News from the 3' end.RNA biology · 2016Article
- Identification of distinct biological functions for four 3'-5' RNA polymerases.Nucleic acids research · 2016Article
- From end to end: tRNA editing at 5'- and 3'-terminal positions.International journal of molecular sciences · 2014Review
- Mitochondrial tRNA 5'-editing in Dictyostelium discoideum and Polysphondylium pallidum.The Journal of biological chemistry · 2014Article
- Strikingly bacteria-like and gene-rich mitochondrial genomes throughout jakobid protists.Genome biology and evolution · 2013Article
- Doing it in reverse: 3'-to-5' polymerization by the Thg1 superfamily.RNA (New York, N.Y.) · 2012Review
- Determinants of tRNA editing and modification: avoiding conundrums, affecting function.Seminars in cell & developmental biology · 2012Review
- tRNAs marked with CCACCA are targeted for degradation.Science (New York, N.Y.) · 2011Article
- When you can't trust the DNA: RNA editing changes transcript sequences.Cellular and molecular life sciences : CMLS · 2011Review
- Genomic organization of eukaryotic tRNAs.BMC genomics · 2010Article
- Two forms of RNA editing are required for tRNA maturation in Physarum mitochondria.RNA (New York, N.Y.) · 2010Article
- Improved tRNA prediction in the American house dust mite reveals widespread occurrence of extremely short minimal tRNAs in acariform mites.BMC genomics · 2009Article
- Origin, evolution, and mechanism of 5' tRNA editing in chytridiomycete fungi.RNA (New York, N.Y.) · 2004Article
- Loss of the mRNA-like region in mitochondrial tmRNAs of jakobids.RNA (New York, N.Y.) · 2004Article
- Evolutionary resealing of a split RNA: Reversal of gene permutation.RNA (New York, N.Y.) · 2004Article
- Controlling translation via modulation of tRNA levels.Wiley interdisciplinary reviews. RNAReview
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The jakobid flagellates are bacteriovorus protists with mitochondrial genomes that are the most ancestral identified to date, in that they most resemble the genomes of the alpha-proteobacterial ancestors of the mitochondrion. Because of the bacterial character of jakobid mitochondrial genomes, it was expected that mechanisms for gene expression and RNA structures would be bacterial in nature. However, sequencing of the mitochondrial genome of the jakobid Seculamonas ecuadoriensis revealed several apparent mismatches in the acceptor stems of two predicted tRNAs. To investigate this observation, we determined the cDNA sequences of these tRNAs by RT-PCR. Our results show that the last three positions of the 3' extremity, plus the discriminator position of seryl and glutamyl tRNAs, are altered posttranscriptionally, restoring orthodox base-pairing and replacing the discriminator with an adenosine residue, in an editing process that resembles that of the metazoan Lithobius forficatus. However, the most 5' of the edited nucleotides is occasionally left unedited, indicating that the editing mechanism proceeds initially by exonucleolytic degradation, followed by repair of the degraded region. This 3' tRNA editing mechanism is likely distinct from that of L. forficatus, despite the apparent similarities between the two systems.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.