ArticleInvestigative ophthalmology & visual science2004
Differential regulation of components of the ubiquitin-proteasome pathway during lens cell differentiation.
Article in Investigative ophthalmology & visual science, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 42 citations in OpenAlex.
- The Putative E3 Ubiquitin Ligase TEX1 Is Required for Nuclear Biology and Developmental Progression ofCells · 2026Article
- Repurposing the Pathogen Box compounds for identification of potent anti-malarials against blood stages of Plasmodium falciparum with PfUCHL3 inhibitory activity.Scientific reports · 2022Article
- Mechanism and Disease Association With a Ubiquitin Conjugating E2 Enzyme: UBE2L3.Frontiers in immunology · 2022Review
- Stabilization of p27FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Connexin Controls Cell-Cycle Exit and Cell Differentiation by Directly Promoting Cytosolic Localization and Degradation of E3 Ligase Skp2.Developmental cell · 2015Article
- Chromatin features, RNA polymerase II and the comparative expression of lens genes encoding crystallins, transcription factors, and autophagy mediators.Molecular vision · 2015Article
- The ubiquitin system: an essential component to unlocking the secrets of malaria parasite biology.Molecular bioSystems · 2014Review
- Newborn mouse lens proteome and its alteration by lysine 6 mutant ubiquitin.Journal of proteome research · 2014Article
- Amniotic membrane-derived cells inhibit proliferation of cancer cell lines by inducing cell cycle arrest.Journal of cellular and molecular medicine · 2012Article
- Proteomic analysis of SRA01/04 transfected with wild-type and mutant HSF4b identified from a Chinese congenital cataract family.Molecular vision · 2012Article
- Development and use of the lens epithelial explant system to study lens differentiation and cataractogenesis.Progress in retinal and eye research · 2010Review
- Gene profiles between non-invasive and invasive colon cancer using laser microdissection and polypeptide analysis.World journal of gastroenterology · 2008Article
- Expression of K6W-ubiquitin in lens epithelial cells leads to upregulation of a broad spectrum of molecular chaperones.Molecular vision · 2008Article
- Molecular mechanisms of paclitaxel and NM-3 on human gastric cancer in a severe combined immune deficiency mice orthotopic implantation model.World journal of gastroenterology · 2007Article
- The anaphase-promoting complex coordinates initiation of lens differentiation.Molecular biology of the cell · 2007Article
- Comparison of gene expression profiles between primary tumor and metastatic lesions in gastric cancer patients using laser microdissection and cDNA microarray.World journal of gastroenterology · 2006Article
- Apoptosis mechanisms of human gastric cancer cell line MKN-45 infected with human mutant p27.World journal of gastroenterology · 2005Article
- Subcellular redistribution of components of the ubiquitin-proteasome pathway during lens differentiation and maturation.Investigative ophthalmology & visual science · 2005Article
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
purposeTo investigate the role for the ubiquitin-proteasome pathway in controlling lens cell proliferation and differentiation and the regulation of the ubiquitin conjugation machinery during the differentiation process.
methodsbFGF-induced lens cell proliferation and differentiation was monitored in rat lens epithelial explants by bromodeoxyuridine (BrdU) incorporation and expression of crystallins and other differentiation markers. Levels of typical substrates for the ubiquitin-proteasome pathway, p21(WAF) and p27(Kip), were monitored during the differentiation process, as were levels and activities of the enzymes involved in ubiquitin conjugation.
resultsExplants treated with bFGF initially underwent enhanced proliferation as indicated by BrdU incorporation. Then they withdrew from the cell cycle as indicated by diminished BrdU incorporation and accumulation of p21(WAF) and p27(Kip). bFGF-induced cell proliferation was prohibited or delayed by proteasome inhibitors. Lens epithelial explants treated with bFGF for 7 days displayed characteristics of lens fibers, including expression of large quantities of crystallins. Whereas levels of E1 remained constant during the differentiation process, the levels of ubiquitin-conjugating enzyme (Ubc)-1 increased approximately twofold, and the thiol ester form of Ubc1 increased approximately threefold on 7 days of bFGF treatment. Levels of Ubc2 increased moderately on bFGF treatment, and most of the Ubc2 was found in the thiol ester form. Although levels of total Ubc3 and -7 remained unchanged, the proportions of Ubc3 and -7 in the thiol ester form were significantly higher in the bFGF-treated explants. Levels of Ubc4/5 and -9 also increased significantly on treatment with bFGF, and more than 90% of Ubc9 was found in the thiol ester form in the bFGF-treated explants. In contrast, levels of Cul1, the backbone of the SCF type of E3s, decreased 50% to 70% in bFGF-treated explants.
conclusionsThe data show that proteolysis through the ubiquitin-proteasome pathway is required for bFGF-induced lens cell proliferation and differentiation. Various components of the ubiquitin-proteasome pathway are differentially regulated during lens cell differentiation. The downregulation of Cul1 appears to contribute to the accumulation of p21(WAF) and p27(Kip), which play an important role in establishing a differentiated phenotype.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.