ArticleJournal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract2004
Kupffer cell-derived Fas ligand plays a role in liver injury and hepatocyte death.
Article in Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 31 citations in OpenAlex.
- Effect of JAK2/STAT3 signaling pathway on liver injury associated with severe acute pancreatitis in rats.Experimental and therapeutic medicine · 2018Article
- Interleukin-35 Attenuates D-Galactosamine/Lipopolysaccharide-Induced Liver Injury via Enhancing Interleukin-10 Production in Kupffer Cells.Frontiers in pharmacology · 2018Article
- Unlike Th1, Th17 cells mediate sustained autoimmune inflammation and are highly resistant to restimulation-induced cell death.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Protein kinase C-zeta (PKC-zeta) regulates Kupffer cell apoptosis during experimental sepsis.Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract · 2007Article
- FasL expression in hepatic antigen-presenting cells and phagocytosis of apoptotic T cells by FasL+ Kupffer cells are indicators of rejection activity in human liver allografts.The American journal of pathology · 2007Article
- Protein kinase C-zeta is critical in pancreatitis-induced apoptosis of Kupffer cells.Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract · 2007Article
- The role of p65 NF-kappaB/RelA in pancreatitis-induced Kupffer cell apoptosis.Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract · 2006Article
- Proteinase-activated receptor-1 mediates elastase-induced apoptosis of human lung epithelial cells.American journal of respiratory cell and molecular biology · 2005Article
- Fas/FasL play a central role in pancreatitis-induced hepatocyte apoptosis.Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract · 2005Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Liver injury is an important prognostic indicator during acute pancreatitis. The aim of this study was to determine the role of Fas ligand (FasL) in hepatocyte injury. Liver parenchymal enzymes were measured in cocultures of hepatocytes and Kupffer cells treated with elastase. FasL and FasL mRNA were measured in elastase-treated Kupffer cells. Hepatocytes were treated with FasL and their viability was assessed by monotetrazolium (MTT), apoptosis by flow cytometry, as well as caspase-3 and p38-mitogen-activated protein kinase (MAPK) by immunoblotting. Elastase increased aspartate aminotransferase and lactate dehydrogenase in cocultures of hepatocyte and Kupffer cells (P<0.040). Elastase increased FasL production from Kupffer cells (P=0.02) and upregulated FasL mRNA (FasL/beta-2 microglobulin (BMG): 0.23+/-0.03 vs. 0.11+/-0.003; P=0.04). FasL increased alanine aminotransferase and lactate dehydrogenase (P<0.03) and reduced hepatocyte viability by 45% (P=0.01). FasL increased the number of dually labeled cells with AnnexinV/7AAD (P=0.03) while upregulating cleavage of caspase-3 and the phosphorylation of p38-MAPK. FasL antibody attenuated the FasL-related increase in dually labeled cells (P=0.02), the cleavage of caspase-3, and phosphorylation of p38-MAPK. Pancreatic elastase upregulates FasL within Kupffer cells. FasL induces hepatocyte injury and death and upregulates p38-MAPK and caspase-3 within hepatocytes. The ability to manipulate interactions between Kupffer cells and hepatocytes may have important therapeutic implications.
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