ArticleCurrent genetics2004
Shuttle vectors for Candida albicans: control of plasmid copy number and elevated expression of cloned genes.
Article in Current genetics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Evaluation of virulence factors and azole resistance mechanisms ofIranian journal of microbiology · 2023Article
- Transcription factor CCG-8 plays a pivotal role in azole adaptive responses of Neurospora crassa by regulating intracellular azole accumulation.Current genetics · 2019Article
- Regulation of ERG3, ERG6, and ERG11 Genes in Antifungal-Resistant isolates of Candida parapsilosisIranian biomedical journal · 2017Article
- Transcription factor CCG-8 as a new regulator in the adaptation to antifungal azole stress.Antimicrobial agents and chemotherapy · 2014Article
- Sterol C-22 Desaturase ERG5 Mediates the Sensitivity to Antifungal Azoles in Neurospora crassa and Fusarium verticillioides.Frontiers in microbiology · 2013Article
- A defect in iron uptake enhances the susceptibility of Cryptococcus neoformans to azole antifungal drugs.Fungal genetics and biology : FG & B · 2012Article
- Potent synergistic effect of doxycycline with fluconazole against Candida albicans is mediated by interference with iron homeostasis.Antimicrobial agents and chemotherapy · 2012Article
- Regulatory circuitry governing fungal development, drug resistance, and disease.Microbiology and molecular biology reviews : MMBR · 2011Review
- Stress, drugs, and evolution: the role of cellular signaling in fungal drug resistance.Eukaryotic cell · 2008Review
- Genotypic evolution of azole resistance mechanisms in sequential Candida albicans isolates.Eukaryotic cell · 2007Article
- Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plasmids containing the inosine monophosphate dehydrogenase gene CaIMH3 from Candida albicans strain ATCC 32354 transform their host to resistance against mycophenolic acid (MPA). The transformants maintain the plasmids at a high copy number (20-40 per cell) and express the CaIMH3 gene at very high levels relative to untransformed controls. The plasmid copy number can be controlled by the concentration of MPA in the media. The transformation procedure is reproducible and the efficiency of transformation is high, up to 15,000 per microgram. Unrearranged plasmids are readily recovered by transforming total DNA from transformants back into Escherichia coli. C. albicans genes cloned into the plasmid are expressed at elevated levels relative to untransformed controls. A derivative vector containing the CaMAL2 promoter and termination sequences expresses the CaERG11 ORF at high levels and confers moderate resistance to fluconazole. These shuttle vectors should facilitate global genomics approaches in C. albicans that have been hampered by its diploid genome.
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Registered trials
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