ArticleThe Biochemical journal2004
Dynamic profiling of the glucose metabolic network in fasted rat hepatocytes using [1,2-13C2]glucose.
Article in The Biochemical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- The Effect of Flavonoids and Topiramate on Glucose Carbon Metabolism in a HepG2 Steatosis Cell Culture Model: A Stable Isotope Study.Nutrients · 2025Article
- Advancements in CHO metabolomics: techniques, current state and evolving methodologies.Frontiers in bioengineering and biotechnology · 2024Review
- Metformin lowers glucose 6-phosphate in hepatocytes by activation of glycolysis downstream of glucose phosphorylation.The Journal of biological chemistry · 2020Article
- The landscape of tiered regulation of breast cancer cell metabolism.Scientific reports · 2019Article
- Acute metabolic responses to high fructose corn syrup ingestion in adolescents with overweight/obesity and diabetes.Journal of nutrition & intermediary metabolism · 2018Article
- HepatoDyn: A Dynamic Model of Hepatocyte Metabolism That Integrates 13C Isotopomer Data.PLoS computational biology · 2016Article
- The Warburg effect: a balance of flux analysis.Metabolomics : Official journal of the Metabolomic Society · 2015Article
- Fructose Alters Intermediary Metabolism of Glucose in Human Adipocytes and Diverts Glucose to Serine Oxidation in the One-Carbon Cycle Energy Producing Pathway.Metabolites · 2015Article
- Regulation of mammalian nucleotide metabolism and biosynthesis.Nucleic acids research · 2015Review
- (13)C metabolic flux analysis shows that resistin impairs the metabolic response to insulin in L6E9 myotubes.BMC systems biology · 2014Article
- Metabolic response of perfused livers to various oxygenation conditions.Biotechnology and bioengineering · 2011Article
- Assessment of usnic acid toxicity in rat primary hepatocytes using ¹³C isotopomer distribution analysis of lactate, glutamate and glucose.Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association · 2011Article
- Compartmentation of glycogen metabolism revealed from 13C isotopologue distributions.BMC systems biology · 2011Article
- Article
- Synergistic activity of histone deacetylase and proteasome inhibition against pancreatic and hepatocellular cancer cell lines.Anticancer research · 2011Article
- Bioactive food components and cancer-specific metabonomic profiles.Journal of biomedicine & biotechnology · 2011Review
- Tracer-based metabolomics: concepts and practices.Clinical biochemistry · 2010Review
- Edelfosine-induced metabolic changes in cancer cells that precede the overproduction of reactive oxygen species and apoptosis.BMC systems biology · 2010Article
- Extending the breadth of metabolite profiling by gas chromatography coupled to mass spectrometry.Trends in analytical chemistry : TRAC · 2008Article
- Global metabolic effects of glycerol kinase overexpression in rat hepatoma cells.Molecular genetics and metabolism · 2008Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Recent studies in metabolic profiling have underscored the importance of the concept of a metabolic network of pathways with special functional characteristics that differ from those of simple reaction sequences. The characterization of metabolic functions requires the simultaneous measurement of substrate fluxes of interconnecting pathways. Here we present a novel stable isotope method by which the forward and reverse fluxes of the futile cycles of the hepatic glucose metabolic network are simultaneously determined. Unlike previous radio-isotope methods, a single tracer [1,2-13C2]D-glucose and mass isotopomer analysis is used. Changes in fluxes of substrate cycles, in response to several gluconeogenic substrates, in isolated fasted hepatocytes from male Wistar rats were measured simultaneously. Incubation with these substrates resulted in a change in glucose-6-phosphatase/glucokinase and glycolytic/gluconeogenic flux ratios. Different net redistributions of intermediates in the glucose network were observed, resulting in distinct metabolic phenotypes of the fasted hepatocytes in response to each substrate condition. Our experimental observations show that the constraints of concentrations of shared intermediates, and enzyme kinetics of intersecting pathways of the metabolic network determine substrate redistribution throughout the network when it is perturbed. These results support the systems-biology notion that network analysis provides an integrated view of the physiological state. Interaction between metabolic intermediates and glycolytic/gluconeogenic pathways is a basic element of cross-talk in hepatocytes, and may explain some of the difficulties in genotype and phenotype correlation.
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Registered trials
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