ArticlePharmaceutical research2004
In situ ileal absorption of insulin in rats: effects of hyaluronidase pretreatment diminishing the mucous/glycocalyx layers.
Article in Pharmaceutical research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed, 34 citations in OpenAlex.
- Neutrophil-derived heparin binding protein triggers vascular leakage and synergizes with myeloperoxidase at the early stage of severe burns (With video).Burns & trauma · 2021Article
- Intestinal extracellular vesicles are altered by vertical sleeve gastrectomy.American journal of physiology. Gastrointestinal and liver physiology · 2021Article
- Region-Dependent Role of Cell-Penetrating Peptides in Insulin Absorption Across the Rat Small Intestinal Membrane.The AAPS journal · 2015Article
- Insulin-loaded nanoparticles based on N-trimethyl chitosan: in vitro (Caco-2 model) and ex vivo (excised rat jejunum, duodenum, and ileum) evaluation of penetration enhancement properties.AAPS PharmSciTech · 2010Article
- Complexation hydrogels for intestinal delivery of interferon beta and calcitonin.Journal of controlled release : official journal of the Controlled Release Society · 2009Article
- Novel oral insulin delivery systems based on complexation polymer hydrogels: single and multiple administration studies in type 1 and 2 diabetic rats.Journal of controlled release : official journal of the Controlled Release Society · 2006Article
- Novel mucosal insulin delivery systems based on fusogenic liposomes.Pharmaceutical research · 2006Article
- Region-dependent role of the mucous/glycocalyx layers in insulin permeation across rat small intestinal membrane.Pharmaceutical research · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo test the hypothesis that the ileal mucous/glycocalyx layers can be removed by hyaluronidase and that their significant roles in insulin absorption can thereby be identified.
methodsRat ileal segments were pretreated with various concentrations of hyaluronidase by "perfusion" or "exposure", and the absorption of insulin and 4.4-, 20-, and 40-kDa fluorescein isothiocyanate-labeled dextrans (FDs) were studied in the in situ ileal loop system. Diminished mucous/glycocalyx layers following the hyaluronidase pretreatment was confirmed by transmission electron microscopy (TEM), whereas intra- and intercellular integrity and/or damage was examined by light microscopy, lactate dehydrogenase (LDH) leakage, and membrane electrical resistance (Rm).
resultsHyaluronidase "perfusion" pretreatment at concentrations > or = 160 U/ml for 30 min significantly increased the hypoglycemic responses following in situ administration of insulin at 50 IU/kg. This enhancing effect was found to be dependent on hyaluronidase concentration and "exposure" periods and accompanied by the TEM observation of diminished mucous/glycocalyx layers from the hyaluronidase pretreatment, yet causing undetectable histological damage. In contrast, the absorption of FDs and the values for LDH leakage and Rm were unaffected by the hyaluronidase pretreatment, suggesting that the layers functioned insignificantly to diffusive absorption.
conclusionsHyaluronidase pretreatment increased ileal absorption of insulin, but not FDs, by virtue of the diminished mucous/glycocalyx layers without causing detectable cellular damage.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.