ArticleThe Plant cell2004
The Arabidopsis chloroplastic NifU-like protein CnfU, which can act as an iron-sulfur cluster scaffold protein, is required for biogenesis of ferredoxin and photosystem I.
Article in The Plant cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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63 citing papers in PubMed, 142 citations in OpenAlex.
- On the non-oxygenic origins of thylakoids.Communications biology · 2025Review
- Functional Relationships of Two NFU Proteins in Maintaining the Abundances of Mitochondrial Iron-Sulfur Proteins.Plant direct · 2025Article
- Hypothetical chloroplast reading frame 51 encodes a photosystem I assembly factor in cyanobacteria.The Plant cell · 2024Article
- Modulation of the wheat transcriptome by TaZFP13D under well-watered and drought conditions.Plant molecular biology · 2024Article
- Research progress on the biosynthesis and delivery of iron-sulfur clusters in the plastid.Plant cell reports · 2023Review
- Protein lipoylation in mitochondria requires Fe-S cluster assembly factors NFU4 and NFU5.Plant physiology · 2022Article
- The DnaJ proteins DJA6 and DJA5 are essential for chloroplast iron-sulfur cluster biogenesis.The EMBO journal · 2021Article
- The evolutionary conserved iron-sulfur protein TCR controls P700 oxidation in photosystem I.iScience · 2021Article
- Functional relationships of three NFU proteins in the biogenesis of chloroplastic iron-sulfur clusters.Plant direct · 2021Article
- [4Fe-4S] cluster trafficking mediated byThe Journal of biological chemistry · 2020Article
- A Global Proteomic Approach Sheds New Light on Potential Iron-Sulfur Client Proteins of the Chloroplastic Maturation Factor NFU3.International journal of molecular sciences · 2020Article
- Regulation of Iron Homeostasis and Use in Chloroplasts.International journal of molecular sciences · 2020Review
- The plastidialThe Journal of biological chemistry · 2020Article
- Physiological and Proteomic Insights Into Red and Blue Light-Mediated Enhancement ofFrontiers in plant science · 2020Article
- A Unique Cytoplasmic-Nuclear Interaction in Sunflower (Frontiers in plant science · 2020Article
- Roles and maturation of iron-sulfur proteins in plastids.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2018Review
- Iron-sulfur protein maturation in Helicobacter pylori: identifying a Nfu-type cluster carrier protein and its iron-sulfur protein targets.Molecular microbiology · 2018Article
- Function and maturation of the Fe-S center in dihydroxyacid dehydratase fromThe Journal of biological chemistry · 2018Article
- Article
- Differential Diel Translation of Transcripts With Roles in the Transfer and Utilization of Iron-Sulfur Clusters in Arabidopsis.Frontiers in plant science · 2018Article
3 more citing papers are in PubMed but not listed here.
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6 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The biosynthesis of iron-sulfur clusters is a highly regulated process involving several proteins. Among them, so-called scaffold proteins play pivotal roles in both the assembly and delivery of iron-sulfur clusters. Here, we report the identification of two chloroplast-localized NifU-like proteins, AtCnfU-V and AtCnfU-IVb, from Arabidopsis (Arabidopsis thaliana) with high sequence similarity to a cyanobacterial NifU-like protein that was proposed to serve as a molecular scaffold. AtCnfU-V is constitutively expressed in several tissues of Arabidopsis, whereas the expression of AtCnfU-IVb is prominent in the aerial parts. Mutant Arabidopsis lacking AtCnfU-V exhibited a dwarf phenotype with faint pale-green leaves and had drastically impaired photosystem I accumulation. Chloroplasts in the mutants also showed a decrease in both the amount of ferredoxin, a major electron carrier of the stroma that contains a [2Fe-2S] cluster, and in the in vitro activity of iron-sulfur cluster insertion into apo-ferredoxin. When expressed in Escherichia coli cells, AtCnfU-V formed a homodimer carrying a [2Fe-2S]-like cluster, and this cluster could be transferred to apo-ferredoxin in vitro to form holo-ferredoxin. We propose that AtCnfU has an important function as a molecular scaffold for iron-sulfur cluster biosynthesis in chloroplasts and thereby is required for biogenesis of ferredoxin and photosystem I.
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