ArticleGene therapy2004
Efficient lentiviral transduction of Herpesvirus saimiri immortalized T cells as a model for gene therapy in primary immunodeficiencies.
Article in Gene therapy, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Article
- Article
- SARS-CoV-2 neutralizing antibodies: Longevity, breadth, and evasion by emerging viral variants.PLoS medicine · 2021Article
- Gene therapy with mesenchymal stem cells expressing IFN-ß ameliorates neuroinflammation in experimental models of multiple sclerosis.British journal of pharmacology · 2017Article
- Absence of WASp Enhances Hematopoietic and Megakaryocytic Differentiation in a Human Embryonic Stem Cell Model.Molecular therapy : the journal of the American Society of Gene Therapy · 2016Article
- Gene Therapy Corrects Mitochondrial Dysfunction in Hematopoietic Progenitor Cells and Fibroblasts from Coq9R239X Mice.PloS one · 2016Article
- Mesenchymal stromal cells express GARP/LRRC32 on their surface: effects on their biology and immunomodulatory capacity.Stem cells (Dayton, Ohio) · 2015Article
- Islet-1 promotes the cardiac-specific differentiation of mesenchymal stem cells through the regulation of histone acetylation.International journal of molecular medicine · 2014Article
- Use of zinc-finger nucleases to knock out the WAS gene in K562 cells: a human cellular model for Wiskott-Aldrich syndrome.Disease models & mechanisms · 2013Article
- Specific marking of hESCs-derived hematopoietic lineage by WAS-promoter driven lentiviral vectors.PloS one · 2012Article
- Development of an all-in-one lentiviral vector system based on the original TetR for the easy generation of Tet-ON cell lines.PloS one · 2011Article
- Dendritic cells transduced with lentiviral vectors expressing VIP differentiate into VIP-secreting tolerogenic-like DCs.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- Improved lentiviral vectors for Wiskott-Aldrich syndrome gene therapy mimic endogenous expression profiles throughout haematopoiesis.Gene therapy · 2008Article
- Lentiviral vectors transcriptionally targeted to hematopoietic cells by WASP gene proximal promoter sequences.Gene therapy · 2005Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Infection of human T lymphocytes with the Herpesvirus saimiri (HVS) yields immortalized T-cell lines (HVS-T) which retain all the phenotypical and functional characteristics of their parental cells. This represents a new experimental model for studying genetic disorders of T lymphocytes. In spite of the efforts of many laboratories, no satisfactory way has been found so far to modify HVS-T cells genetically. We have analyzed the capacity of oncoretroviral (MLV)- and lentiviral (HIV-1)-based vectors pseudotyped with vesicular stomatitis virus glycoprotein (VSVg) to transduce HVS-T cells. HIV-1-derived vectors efficiently transduced HVS-T cell lines, reaching up to 85% of cells expressing the transgene in a single round of infection. MLV-based vectors, on the other hand, were unable to transduce more than 1% of any of the HVS-T cell lines analyzed. Lentiviral-driven gene expression was maintained constant and stable in HVS-T cells for a minimum of 48 days. We also observed that although the lentiviral transduction efficiency achieved on HVS-T cells is lower than that obtained with tumor or primary endothelial cells, it is nevertheless similar to that found with activated primary T cells.
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