Evidence map›Paper›PMID 15028697›Full record

ArticleJournal of bacteriology2004

Sequence polymorphism in the glycosylation island and flagellins of Pseudomonas aeruginosa.

Shiwani K Arora, Matthew C Wolfgang, Stephen Lory, Reuben Ramphal

Open access · greenAbstract read
In one paragraph

Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
3.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 58 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Article
  6. Mutational Evolution ofFrontiers in genetics · 2018
    Article
  7. Article
  8. Article
  9. Article
  10. The sweet tooth of bacteria: common themes in bacterial glycoconjugates.Microbiology and molecular biology reviews : MMBR · 2014
    Review
  11. Gram-negative flagella glycosylation.International journal of molecular sciences · 2014
    Review
  12. Article
  13. Article
  14. Article
  15. Article
  16. The accessory genome of Pseudomonas aeruginosa.Microbiology and molecular biology reviews : MMBR · 2010
    Review
  17. Protein glycosylation in bacteria: sweeter than ever.Nature reviews. Microbiology · 2010
    Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Shiwani K AroraDepartment of Medicine and Infectious Diseases, University of Florida, Gainesville, Florida 32610, USA.
Matthew C Wolfgang
Stephen Lory
Reuben Ramphal
Harvard University · USUniversity of Florida · US

Funding

FLAGELLIN GLYCOSYLATION AND VIRULENCE OF P. AEROGINOSAR01AI047852 · NIAID · UNIVERSITY OF FLORIDA · PI RAMPHAL, REUBEN · 2001 to 2005
$1.4M
NIAID NIH HHS AI 47852NIAID NIH HHS R01 AI047852
6 · The paper itself

Abstract

A genomic island consisting of 14 open reading frames, orfA to orfN was previously identified in Pseudomonas aeruginosa strain PAK and shown to be essential for glycosylation of flagellin. DNA microarray hybridization analysis of a number of P. aeruginosa strains from diverse origins showed that this island is polymorphic. PCR and sequence analysis confirmed that many P. aeruginosa strains carry an abbreviated version of the island (short island) in which orfD, -E and -H are polymorphic and orfI, -J, -K, -L, and -M are absent. To ascertain whether there was a relationship between the inheritance of the short island and specific flagellin sequence variants, complete or partial nucleotide sequences of flagellin genes from 24 a-type P. aeruginosa strains were determined. Two distinct flagellin subtypes, designated A1 and A2, were apparent. Strains with the complete 14-gene island (long island) were almost exclusively of the A1 type, whereas strains carrying the short island were associated with both A1- and A2-type flagellins. These findings indicate that P. aeruginosa possesses a relatively low number of distinct flagellin types and probably has the capacity to further diversify this antigenic surface protein by glycosylation.

Indexed as

Amino Acid SequencePolymorphism, GeneticBacterial ProteinsFlagellinGlycosylationHumansMolecular Sequence DataOligonucleotide Array Sequence AnalysisPseudomonas aeruginosaPseudomonas InfectionsSequence Analysis, DNABacterial ProteinsFlagellin

Identifiers

PMID15028697
PMCPMC374406
OpenAlexW2172026438

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.