ArticleJournal of bacteriology2004
A conjugation-based system for genetic analysis of group II intron splicing in Lactococcus lactis.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 14 citations in OpenAlex.
- Group II Introns Generate Functional Chimeric Relaxase Enzymes with Modified Specificities through Exon Shuffling at Both the RNA and DNA Level.Molecular biology and evolution · 2021Article
- Circularization pathway of a bacterial group II intron.Nucleic acids research · 2016Article
- Novel system for efficient isolation of Clostridium double-crossover allelic exchange mutants enabling markerless chromosomal gene deletions and DNA integration.Applied and environmental microbiology · 2012Article
- Isolation and characterization of functional tripartite group II introns using a Tn5-based genetic screen.PloS one · 2012Article
- Contribution of base-pairing interactions between group II intron fragments during trans-splicing in vivo.RNA (New York, N.Y.) · 2011Article
- Splicing of the Sinorhizobium meliloti RmInt1 group II intron provides evidence of retroelement behavior.Nucleic acids research · 2011Article
- Distribution, diversity, and potential mobility of extrachromosomal elements related to the Bacillus anthracis pXO1 and pXO2 virulence plasmids.Applied and environmental microbiology · 2009Article
- Specificity determinants of conjugative DNA processing in the Enterococcus faecalis plasmid pCF10 and the Lactococcus lactis plasmid pRS01.Molecular microbiology · 2007Article
- Trans-splicing of the Ll.LtrB group II intron in Lactococcus lactis.Nucleic acids research · 2007Article
- Genetic characterization of the conjugative DNA processing system of enterococcal plasmid pCF10.Plasmid · 2006Article
- Quantitative analysis of group II intron expression and splicing in Lactococcus lactis.Applied and environmental microbiology · 2005Article
- Conjugative transfer of the Lactococcus lactis chromosomal sex factor promotes dissemination of the Ll.LtrB group II intron.Journal of bacteriology · 2005Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The conjugative element pRS01 from Lactococcus lactis encodes the putative relaxase protein LtrB. The ltrB gene is interrupted by the functional group II intron Ll.ltrB. Accurate splicing of the two ltrB exons is required for synthesis of the mRNA encoding the LtrB conjugative relaxase and subsequent plasmid transfer. A conjugation-based genetic assay was developed to identify Ll.ltrB mutations that affect splicing. In this assay a nonsplicing, transfer-defective pRS01 derivative (pM1014) and a shuttle vector carrying the ltrB region, including the Ll.ltrB intron (pCOM9), are used. pCOM9 provides splicing-dependent complementation of the transfer defect of pM1014. Site-directed mutations within Ll.ltrB, either in the catalytic RNA or in the intron-encoded protein gene ltrA, were generated in the context of pCOM9. When these mutants were tested in the conjugation-based assay, significantly reduced mating was observed. Quantitative molecular analysis of in vivo splicing activity confirmed that the observed mating defects resulted from reduced splicing. Once the system was validated for the engineered mutants, random mutagenesis of the intron followed by genetic and molecular screening for splicing defects resulted in identification of point mutations that affect splicing.
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