ArticleBMC genomics2004
Identification of Bacillus anthracis specific chromosomal sequences by suppressive subtractive hybridization.
Article in BMC genomics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 21 citations in OpenAlex.
- New bacteriophage-derived lysins, LysJ and LysF, with the potential to control Bacillus anthracis.Applied microbiology and biotechnology · 2024Article
- Phages preying on Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis: past, present and future.Viruses · 2014Review
- Divergence of protein-coding capacity and regulation in the Bacillus cereus sensu lato group.BMC bioinformatics · 2014Article
- Existence of separate domains in lysin PlyG for recognizing Bacillus anthracis spores and vegetative cells.Antimicrobial agents and chemotherapy · 2012Article
- Characterization of an environmental strain of Bacillus thuringiensis from a hot spring in Western Himalayas.Current microbiology · 2011Article
- Proteomic profiling and identification of immunodominant spore antigens of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis.Applied and environmental microbiology · 2006Article
- Detailed genomic analysis of the Wbeta and gamma phages infecting Bacillus anthracis: implications for evolution of environmental fitness and antibiotic resistance.Journal of bacteriology · 2006Article
- A new Bacillus anthracis found in wild chimpanzees and a gorilla from West and Central Africa.PLoS pathogens · 2006Article
- Real-time PCR assay for a unique chromosomal sequence of Bacillus anthracis.Journal of clinical microbiology · 2004Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBacillus anthracis, Bacillus thuringiensis and Bacillus cereus are closely related members of the B. cereus-group of bacilli. Suppressive subtractive hybridization (SSH) was used to identify specific chromosomal sequences unique to B. anthracis.
resultsTwo SSH libraries were generated. Genomic DNA from plasmid-cured B. anthracis was used as the tester DNA in both libraries, while genomic DNA from either B. cereus or B. thuringiensis served as the driver DNA. Progressive screening of the libraries by colony filter and Southern blot analyses identified 29 different clones that were specific for the B. anthracis chromosome relative not only to the respective driver DNAs, but also to seven other different strains of B. cereus and B. thuringiensis included in the process. The nucleotide sequences of the clones were compared with those found in genomic databases, revealing that over half of the clones were located into 2 regions on the B. anthracis chromosome.
conclusionsGenes encoding potential cell wall synthesis proteins dominated one region, while bacteriophage-related sequences dominated the other region. The latter supports the hypothesis that acquisition of these bacteriophage sequences occurred during or after speciation of B. anthracis relative to B. cereus and B. thuringiensis. This study provides insight into the chromosomal differences between B. anthracis and its closest phylogenetic relatives.
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