ArticleMolecular and cellular biology2004
Chromatin-mediated restriction of nuclear factor 1/CTF binding in a repressed and hormone-activated promoter in vivo.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
- Article
- Roles of the NFI/CTF gene family in transcription and development: update 2025.Academia biology · 2025Article
- PIM2-mediated phosphorylation contributes to granulosa cell survival via resisting apoptosis during folliculogenesis.Clinical and translational medicine · 2021Article
- Selective breeding for high anxiety introduces a synonymous SNP that increases neuropeptide S receptor activity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Insulin represses transcription of the thyroid stimulating hormone beta-subunit gene through increased recruitment of nuclear factor I.The Journal of biological chemistry · 2010Article
- Highly compacted chromatin formed in vitro reflects the dynamics of transcription activation in vivo.Molecular cell · 2010Article
- The CAAT-binding transcription factor 1/nuclear factor 1 binding site is important in beta-myosin heavy chain antisense promoter regulation in rats.Experimental physiology · 2009Article
- FoxA1 binding directs chromatin structure and the functional response of a glucocorticoid receptor-regulated promoter.Molecular and cellular biology · 2009Article
- Bacillus anthracis lethal toxin represses MMTV promoter activity through transcription factors.Journal of molecular biology · 2009Article
- Glucocorticoid receptor dynamics and gene regulation.Stress (Amsterdam, Netherlands) · 2009Review
- Nuclear factor 1 and T-cell factor/LEF recognition elements regulate Pitx2 transcription in pituitary development.Molecular and cellular biology · 2007Article
- Mechanism of histone H1-stimulated glucocorticoid receptor DNA binding in vivo.Molecular and cellular biology · 2007Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mouse mammary tumor virus (MMTV) promoter-driven transcription is induced by glucocorticoid hormone via binding of the glucocorticoid receptor (GR). The MMTV promoter also harbors a binding site for nuclear factor 1 (NF1). NF1 and GR were expressed in Xenopus oocytes; this revealed GR-NF1 cooperativity both in terms of DNA binding and chromatin remodeling but not transcription. A fraction of NF1 sites were occupied in a hormone-dependent fashion, but a significant and NF1 concentration-dependent fraction were constitutively bound. Activation of the MMTV promoter resulted in an approximately 50-fold increase in the NF1 accessibility for its DNA site. The hormone-dependent component of NF1 binding was dissociated by addition of a GR antagonist; however, the antagonist RU486, which supports partial GR-DNA binding, also maintained partial NF1 binding. Hence GR-NF1 cooperativity is independent of agonist-driven chromatin remodeling. NF1 induced the formation of a micrococcal-nuclease-resistant protein-DNA complex containing the DNA segment from -185 to -55, the MMTV enhanceosome. Coexpression of NF1 and Oct1 resulted in a significant stimulation of hormone-induced MMTV transcription and also in increased basal transcription. We propose that hormone-independent NF1 binding may be involved in maintaining transcriptional competence and establishment of tissue-specific gene networks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.