ArticleMolecular and cellular biology2004
Modular structure of the human lamin B2 replicator.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 92 citations in OpenAlex.
- Where and when to start: Regulating DNA replication origin activity in eukaryotic genomes.Nucleus (Austin, Tex.) · 2023Review
- Cockayne syndrome group A and ferrochelatase finely tune ribosomal gene transcription and its response to UV irradiation.Nucleic acids research · 2021Article
- Order from clutter: selective interactions at mammalian replication origins.Nature reviews. Genetics · 2017Review
- Genome-wide identification and characterisation of human DNA replication origins by initiation site sequencing (ini-seq).Nucleic acids research · 2016Article
- Distinct epigenetic features of differentiation-regulated replication origins.Epigenetics & chromatin · 2016Article
- Allele-specific analysis of DNA replication origins in mammalian cells.Nature communications · 2015Article
- Peaks cloaked in the mist: the landscape of mammalian replication origins.The Journal of cell biology · 2015Review
- The hunt for origins of DNA replication in multicellular eukaryotes.F1000prime reports · 2015Review
- Epigenetic landscape for initiation of DNA replication.Chromosoma · 2014Review
- Allele-specific genome-wide profiling in human primary erythroblasts reveal replication program organization.PLoS genetics · 2014Article
- G4 motifs affect origin positioning and efficiency in two vertebrate replicators.The EMBO journal · 2014Article
- High-resolution analysis of DNA synthesis start sites and nucleosome architecture at efficient mammalian replication origins.The EMBO journal · 2013Article
- Enhanced and prolonged baculovirus-mediated expression by incorporating recombinase system and in cis elements: a comparative study.Nucleic acids research · 2013Article
- Article
- Mechanisms for initiating cellular DNA replication.Annual review of biochemistry · 2013Review
- The epigenetic control of E-box and Myc-dependent chromatin modifications regulate the licensing of lamin B2 origin during cell cycle.Nucleic acids research · 2012Article
- Prevention of transcriptional silencing by a replicator-binding complex consisting of SWI/SNF, MeCP1, and hnRNP C1/C2.Molecular and cellular biology · 2011Article
- Initiation of DNA replication: functional and evolutionary aspects.Annals of botany · 2011Review
- Defining the replication program through the chromatin landscape.Critical reviews in biochemistry and molecular biology · 2011Review
- Pre-replication complex proteins assemble at regions of low nucleosome occupancy within the Chinese hamster dihydrofolate reductase initiation zone.Nucleic acids research · 2011Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cis-acting elements necessary for the activity of DNA replication origins in metazoan cells are still poorly understood. Here we report a thorough characterization of the DNA sequence requirements of the origin associated with the human lamin B2 gene. A 1.2-kb DNA segment, comprising the start site of DNA replication and located within a large protein-bound region, as well as a CpG island, displays origin activity when moved to different ectopic positions. Genomic footprinting analysis of both the endogenous and the ectopic origins indicates that the large protein complex is assembled in both cases around the replication start site. Replacement of this footprinted region with an unrelated sequence, maintaining the CpG island intact, abolishes origin activity and the interaction with hORC2, a subunit of the origin recognition complex. Conversely, the replacement of 17 bp within the protected region reduces the extension of the protection without affecting the interaction with hORC2. This substitution does not abolish the origin activity but makes it more sensitive to the integration site. Finally, the nearby CpG island positively affects the efficiency of initiation. This analysis reveals the modular structure of the lamin B2 origin and supports the idea that sequence elements close to the replication start site play an important role in origin activation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.