ArticleThe Journal of cell biology2004
In vitro FRAP reveals the ATP-dependent nuclear mobilization of the exon junction complex protein SRm160.
Article in The Journal of cell biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Non-random inter-speckle material transfer mediated by dynamic multiphase interchromatin connections.The EMBO journal · 2026Article
- Assembly of exon junction complexes on spliceosomes begins before spliceosome activation.Nucleic acids research · 2026Article
- Phosphoproteomic and proteomic profiling in post-infarction chronic heart failure.Frontiers in pharmacology · 2023Article
- Stress induced TDP-43 mobility loss independent of stress granules.Nature communications · 2022Article
- Tau aggregates are RNA-protein assemblies that mislocalize multiple nuclear speckle components.Neuron · 2021Article
- HITS-CLIP reveals sex-differential RNA binding and alterative splicing regulation of SRm160 in Drosophila.Journal of molecular cell biology · 2019Article
- Coordinated Dynamics of RNA Splicing Speckles in the Nucleus.Journal of cellular physiology · 2016Article
- Multifunctional RNA processing protein SRm160 induces apoptosis and regulates eye and genital development in Drosophila.Genetics · 2014Article
- Regulation of mRNA export by the PI3 kinase/AKT signal transduction pathway.Molecular biology of the cell · 2013Article
- Article
- Live cell imaging of the cancer-related transcription factor RUNX2 during mitotic progression.Journal of cellular physiology · 2011Article
- Effect of ATP binding and hydrolysis on dynamics of canine parvovirus NS1.Journal of virology · 2010Article
- Subnuclear localization and intranuclear trafficking of transcription factors.Methods in molecular biology (Clifton, N.J.) · 2010Article
- Chromatin remodeling complexes interact dynamically with a glucocorticoid receptor-regulated promoter.Molecular biology of the cell · 2008Article
- Compartmentation of the nucleolar processing proteins in the granular component is a CK2-driven process.Molecular biology of the cell · 2006Article
- In vivo BiFC analysis of Y14 and NXF1 mRNA export complexes: preferential localization within and around SC35 domains.The Journal of cell biology · 2006Article
- The identification and characterization of a testis-specific cDNA during spermatogenesis.Cellular & molecular biology letters · 2006Article
- Mechanism of mRNA transport in the nucleus.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Chinese hamster ORC subunits dynamically associate with chromatin throughout the cell-cycle.Experimental cell research · 2005Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We present a new in vitro system for characterizing the binding and mobility of enhanced green fluorescent protein (EGFP)-labeled nuclear proteins by fluorescence recovery after photobleaching in digitonin-permeabilized cells. This assay reveals that SRm160, a splicing coactivator and component of the exon junction complex (EJC) involved in RNA export, has an adenosine triphosphate (ATP)-dependent mobility. Endogenous SRm160, lacking the EGFP moiety, could also be released from sites at splicing speckled domains by an ATP-dependent mechanism. A second EJC protein, RNPS1, also has an ATP-dependent mobility, but SRm300, a protein that binds to SRm160 and participates with it in RNA splicing, remains immobile after ATP supplementation. This finding suggests that SRm160-containing RNA export, but not splicing, complexes have an ATP-dependent mobility. We propose that RNA export complexes have an ATP-regulated mechanism for release from binding sites at splicing speckled domains. In vitro fluorescence recovery after photobleaching is a powerful tool for identifying cofactors required for nuclear binding and mobility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.