ArticleBritish journal of pharmacology2004
Role of the epithelium and acetylcholine in mediating the contraction to 5-hydroxytryptamine in the mouse isolated trachea.
Article in British journal of pharmacology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 58 citations in OpenAlex.
- 5-HTJournal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi · 2021Article
- Acetylcholine From Tuft Cells: The Updated Insights Beyond Its Immune and Chemosensory Functions.Frontiers in cell and developmental biology · 2020Review
- Interactive Effects of Sertraline and Diphenhydramine on Biochemical and Behavioral Responses in Crucian Carp (International journal of environmental research and public health · 2019Article
- Voltage effects on muscarinic acetylcholine receptor-mediated contractions of airway smooth muscle.Physiological reports · 2018Article
- Caveolin-3 differentially orchestrates cholinergic and serotonergic constriction of murine airways.Scientific reports · 2018Article
- Article
- Caveolin-1: Functional Insights into Its Role in Muscarine- and Serotonin-Induced Smooth Muscle Constriction in Murine Airways.Frontiers in physiology · 2017Article
- Article
- In vitro measurements of tracheal constriction using mice.Journal of visualized experiments : JoVE · 2012Article
- TNF-α-induces airway hyperresponsiveness to cholinergic stimulation in guinea pig airways.British journal of pharmacology · 2012Article
- Muscarinic receptors and their antagonists in COPD: anti-inflammatory and antiremodeling effects.Mediators of inflammation · 2012Review
- Epithelium integrity is crucial for the relaxant activity of brain natriuretic peptide in human isolated bronchi.British journal of pharmacology · 2011Article
- Muscarinic receptor-mediated bronchoconstriction is coupled to caveolae in murine airways.American journal of physiology. Lung cellular and molecular physiology · 2010Article
- Bacterial toxins and the nervous system: neurotoxins and multipotential toxins interacting with neuronal cells.Toxins · 2010Review
- Mast cell-cholinergic nerve interaction in mouse airways.The Journal of physiology · 2009Article
- Cooperation between mast cells and neurons is essential for antigen-mediated bronchoconstriction.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Action of propranolol in the reaction of smooth musculature of tracheal rings induced with acetylcholine, histamine, serotonin (5-HT) and prostaglandin (PGF2-alfa) in vitro and in vivo.Bosnian journal of basic medical sciences · 2009Article
- Serotonin increases cilia-driven particle transport via an acetylcholine-independent pathway in the mouse trachea.PloS one · 2009Article
- The epithelial cholinergic system of the airways.Histochemistry and cell biology · 2008Review
- IL-1beta induces murine airway 5-HT2A receptor hyperresponsiveness via a non-transcriptional MAPK-dependent mechanism.Respiratory research · 2007Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. The 5-HT receptor subtype that mediates bronchocontraction and the involvement of neuronal and non-neuronal acetylcholine was assessed in murine isolated tracheae. 2. Atropine (1-10 nM) caused a rightward shift of the methacholine concentration-effect curves (pA(2)=9.0) but reduced the maximum response to 5-HT, suggesting that 5-HT acts as an indirect agonist. The potency of 5-HT receptor agonists (alpha-methyl-5-HT approximately 5-HT>5-carboxamidotryptamine), together with the competitive antagonism of 5-HT by ketanserin (pA(2)=9.4), suggests the involvement of the 5-HT(2A) receptor. 3. While cholinergic twitch responses to electrical field stimulation were abolished by the fast sodium channel inhibitor tetrodotoxin (300 nM), as well as by combined blockade of N-, P- and Q-type voltage-operated calcium channels by omega-conotoxin GVIA (30 nM) and agatoxin IVA (100 nM), responses to 5-HT were unaffected. Similarly, botulinum toxin A (50 nM) inhibited EFS twitch responses, but not contractions to 5-HT. 4. Choline acetyltransferase immunoreactivity was localised to ganglia and nerve fibres as well as approximately half the epithelial cells in the preparation. Removal of the epithelial layer markedly attenuated the contractile response to 5-HT, but had no effect on contractions to either methacholine or EFS. 5. These findings suggest that 5-HT, acting at 5-HT(2A) receptors on mouse tracheal epithelial cells, stimulates these cells to release acetylcholine, which then causes contraction of airway smooth muscle. This phenomenon should be borne in mind in when interpreting studies of murine models of airway disease.
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