ArticleProceedings of the National Academy of Sciences of the United States of America2004
A nascent polypeptide domain that can regulate translation elongation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
43 citing papers in PubMed, 86 citations in OpenAlex.
- Tiny but mighty: Diverse functions of uORFs that regulate gene expression.Computational and structural biotechnology journal · 2024Review
- Translational regulation by uORFs and start codon selection stringency.Genes & development · 2023Review
- A Conserved uORF Regulates APOBEC3G Translation and Is Targeted by HIV-1 Vif Protein to Repress the Antiviral Factor.Biomedicines · 2021Article
- Translational autoregulation of the S. cerevisiae high-affinity polyamine transporter Hol1.Molecular cell · 2021Article
- Control of translation by eukaryotic mRNA transcript leaders-Insights from high-throughput assays and computational modeling.Wiley interdisciplinary reviews. RNA · 2021Review
- Impacts of uORF codon identity and position on translation regulation.Nucleic acids research · 2019Article
- PausePred and Rfeet: webtools for inferring ribosome pauses and visualizing footprint density from ribosome profiling data.RNA (New York, N.Y.) · 2018Article
- Viral Short ORFs and Their Possible Functions.Proteomics · 2018Review
- Polyamine Control of Translation Elongation Regulates Start Site Selection on Antizyme Inhibitor mRNA via Ribosome Queuing.Molecular cell · 2018Article
- Optimal Down Regulation of mRNA Translation.Scientific reports · 2017Article
- Translation and Co-translational Membrane Engagement of Plastid-encoded Chlorophyll-binding Proteins Are Not Influenced by Chlorophyll Availability in Maize.Frontiers in plant science · 2017Article
- Review
- Ribosome Elongation Stall Directs Gene-specific Translation in the Integrated Stress Response.The Journal of biological chemistry · 2016Article
- Codon Usage Influences the Local Rate of Translation Elongation to Regulate Co-translational Protein Folding.Molecular cell · 2015Article
- An upstream open reading frame is essential for feedback regulation of ascorbate biosynthesis in Arabidopsis.The Plant cell · 2015Article
- Identification of novel Arabidopsis thaliana upstream open reading frames that control expression of the main coding sequences in a peptide sequence-dependent manner.Nucleic acids research · 2015Article
- Regional discrimination and propagation of local rearrangements along the ribosomal exit tunnel.Journal of molecular biology · 2014Article
- A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors.The Journal of biological chemistry · 2014Article
- Sequence selectivity of macrolide-induced translational attenuation.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Translation of CGA codon repeats in yeast involves quality control components and ribosomal protein L1.RNA (New York, N.Y.) · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The evolutionarily conserved fungal arginine attenuator peptide (AAP), as a nascent peptide, stalls the translating ribosome in response to the presence of a high concentration of the amino acid arginine. Here we examine whether the AAP maintains regulatory function in fungal, plant, and animal cell-free translation systems when placed as a domain near the N terminus or internally within a large polypeptide. Pulse-chase analyses of the radiolabeled polypeptides synthesized in these systems indicated that wild-type AAP functions at either position to stall polypeptide synthesis in response to arginine. Toeprint analyses performed to map the positions of stalled ribosomes on transcripts introduced into the fungal system revealed that ribosome stalling required translation of the AAP coding sequence. The positions of the stalled ribosomes were consistent with the sizes of the radiolabeled polypeptide intermediates. These findings demonstrate that an internal polypeptide domain in a nascent chain can regulate eukaryotic translational elongation in response to a small molecule. Apparently the peptide-sensing features are conserved in fungal, plant, and animal ribosomes. These data provide precedents for translational strategies that would allow domains within nascent polypeptide chains to modulate gene expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.