ArticleMolecular biology of the cell2004
Cytoplasmic dynein-dynactin complex is required for spermatid growth but not axoneme assembly in Drosophila.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 51 citations in OpenAlex.
- Article
- A comparison of spermatogenesis between flies and men-conserved processes of male gamete production.Human reproduction update · 2025Review
- Relation between proteome characterization and semen quality in Italian chicken breeds.PloS one · 2025Article
- Mitochondrial Differentiation during Spermatogenesis: Lessons fromInternational journal of molecular sciences · 2024Review
- Article
- Emergent dynamics of adult stem cell lineages from single nucleus and single cell RNA-Seq ofeLife · 2023Article
- Long noncoding RNA regulation of spermatogenesis via the spectrin cytoskeleton in Drosophila.G3 (Bethesda, Md.) · 2021Article
- Wampa is a dynein subunit required for axonemal assembly and male fertility in Drosophila.Developmental biology · 2020Article
- Combover interacts with the axonemal component Rsp3 and is required forDevelopment (Cambridge, England) · 2019Article
- Drosophila Pif1A is essential for spermatogenesis and is the homolog of human CCDC157, a gene associated with idiopathic NOA.Cell death & disease · 2019Article
- Atypical septate junctions maintain the somatic enclosure around maturing spermatids and prevent premature sperm release inBiology open · 2019Article
- Analysis of Drosophila melanogaster testis transcriptome.BMC genomics · 2018Article
- Identification of genetic networks that act in the somatic cells of the testis to mediate the developmental program of spermatogenesis.PLoS genetics · 2017Article
- Dynein light chain 1 (LC8) association enhances microtubule stability and promotes microtubule bundling.The Journal of biological chemistry · 2012Article
- The Drosophila RZZ complex - roles in membrane trafficking and cytokinesis.Journal of cell science · 2012Article
- Drosophila spermiogenesis: Big things come from little packages.Spermatogenesis · 2012Article
- The poly(A) polymerase GLD2 is required for spermatogenesis in Drosophila melanogaster.Development (Cambridge, England) · 2011Article
- Dynein light chain 1 functions in somatic cyst cells regulate spermatogonial divisions in Drosophila.Scientific reports · 2011Article
- Article
- LUMP is a putative double-stranded RNA binding protein required for male fertility in Drosophila melanogaster.PloS one · 2011Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Spermatids derived from a single gonial cell remain interconnected within a cyst and elongate by synchronized growth inside the testis in Drosophila. Cylindrical spectrin-rich elongation cones form at their distal ends during the growth. The mechanism underlying this process is poorly understood. We found that developing sperm tails were abnormally coiled at the growing ends inside the cysts in the Drosophila Dynein light chain 1 (ddlc1) hemizygous mutant testis. A quantitative assay showed that average number of elongation cones was reduced, they were increasingly deformed, and average cyst lengths were shortened in ddlc1 hemizygous testes. These phenotypes were further enhanced by additional partial reduction of Dhc64C and Glued and rescued by Myc-PIN/LC8 expression in the gonial cells in ddlc1 backgrounds. Furthermore, DDLC1, DHC, and GLUED were enriched at the distal ends of growing spermatids. Finally, ultrastructure analysis of ddlc1 testes revealed abnormally formed interspermatid membrane, but the 9 + 2 microtubule organization, the radial spoke structures, and the Dynein arms of the axoneme were normal. Together, these findings suggest that axoneme assembly and spermatid growth involve independent mechanisms in Drosophila and DDLC1 interacts with the Dynein-Dynactin complex at the distal ends of spermatids to maintain the spectrin cytoskeleton assembly and cell growth.
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