ArticleGenetics2004
Reciprocal translocations in Saccharomyces cerevisiae formed by nonhomologous end joining.
Article in Genetics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 45 citations in OpenAlex.
- Origin, evolution, and maintenance of gene-strand bias in bacteria.Nucleic acids research · 2024Article
- Review
- Frequency of DNA end joiningProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Breakpoint mapping and haplotype analysis of translocation t(1;12)(q43;q21.1) in two apparently independent families with vascular phenotypes.Molecular genetics & genomic medicine · 2018Article
- Consider the workhorse: Nonhomologous end-joining in budding yeast.Biochemistry and cell biology = Biochimie et biologie cellulaire · 2016Review
- The non-homologous end-joining pathway of S. cerevisiae works effectively in G1-phase cells, and religates cognate ends correctly and non-randomly.DNA repair · 2016Article
- Sex-determination system in the diploid yeast Zygosaccharomyces sapae.G3 (Bethesda, Md.) · 2014Article
- Article
- Large chromosome deletions, duplications, and gene conversion events accumulate with age in normal human colon crypts.Aging cell · 2013Article
- Yeast pol4 promotes tel1-regulated chromosomal translocations.PLoS genetics · 2013Article
- Warburg effect and translocation-induced genomic instability: two yeast models for cancer cells.Frontiers in oncology · 2012Article
- Msh2 blocks an alternative mechanism for non-homologous tail removal during single-strand annealing in Saccharomyces cerevisiae.PloS one · 2009Article
- Chromosomal translocations caused by either pol32-dependent or pol32-independent triparental break-induced replication.Molecular and cellular biology · 2009Article
- Unexpected complexity at breakpoint junctions in phenotypically normal individuals and mechanisms involved in generating balanced translocations t(1;22)(p36;q13).Genome research · 2008Article
- Double-strand breaks associated with repetitive DNA can reshape the genome.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Proofreading activity of DNA polymerase Pol2 mediates 3'-end processing during nonhomologous end joining in yeast.PLoS genetics · 2008Article
- Retrosequence formation restructures the yeast genome.Genes & development · 2007Article
- Article
- Capture of linear fragments at a double-strand break in yeast.Nucleic acids research · 2007Article
- Global mapping of transposon location.PLoS genetics · 2006Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Reciprocal translocations are common in cancer cells, but their creation is poorly understood. We have developed an assay system in Saccharomyces cerevisiae to study reciprocal translocation formation in the absence of homology. We induce two specific double-strand breaks (DSBs) simultaneously on separate chromosomes with HO endonuclease and analyze the subsequent chromosomal rearrangements among surviving cells. Under these conditions, reciprocal translocations via nonhomologous end joining (NHEJ) occur at frequencies of approximately 2-7 x 10(-5)/cell exposed to the DSBs. Yku80p is a component of the cell's NHEJ machinery. In its absence, reciprocal translocations still occur, but the junctions are associated with deletions and extended overlapping sequences. After induction of a single DSB, translocations and inversions are recovered in wild-type and rad52 strains. In these rearrangements, a nonrandom assortment of sites have fused to the DSB, and their junctions show typical signs of NHEJ. The sites tend to be between open reading frames or within Ty1 LTRs. In some cases the translocation partner is formed by a break at a cryptic HO recognition site. Our results demonstrate that NHEJ-mediated reciprocal translocations can form in S. cerevisiae as a consequence of DSB repair.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.