ArticleGenetics2004
Homology modeling and mutational analysis of Ho endonuclease of yeast.
Article in Genetics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 33 citations in OpenAlex.
- Repeated losses of self-fertility shaped heterozygosity and polyploidy in yeast evolution.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Wild yeast isolation by middle-school students reveals features of populations residing on North American oaks.G3 (Bethesda, Md.) · 2025Article
- Wild yeast isolation by middle school students reveals features of North American oak populations ofbioRxiv : the preprint server for biology · 2024Article
- Lessons from the Nakaseomyces: mating-type switching, DSB repair and evolution of Ho.Current genetics · 2021Review
- Article
- Role of Cdc23/Mcm10 in generating the ribonucleotide imprint at the mat1 locus in fission yeast.Nucleic acids research · 2019Article
- An Evolutionary Perspective on Yeast Mating-Type Switching.Genetics · 2017Review
- Article
- Sex-determination system in the diploid yeast Zygosaccharomyces sapae.G3 (Bethesda, Md.) · 2014Article
- The mitochondrial LSU rRNA group II intron of Ustilago maydis encodes an active homing endonuclease likely involved in intron mobility.PloS one · 2012Article
- Heterothallism in Saccharomyces cerevisiae isolates from nature: effect of HO locus on the mode of reproduction.Molecular ecology · 2010Article
- Characterization of Mycobacterium leprae RecA intein, a LAGLIDADG homing endonuclease, reveals a unique mode of DNA binding, helical distortion, and cleavage compared with a canonical LAGLIDADG homing endonuclease.The Journal of biological chemistry · 2009Article
- Nuclear export of Ho endonuclease of yeast via Msn5.Current genetics · 2008Article
- Homing endonuclease I-TevIII: dimerization as a means to a double-strand break.Nucleic acids research · 2007Article
- The DNA damage-inducible UbL-UbA protein Ddi1 participates in Mec1-mediated degradation of Ho endonuclease.Molecular and cellular biology · 2005Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ho endonuclease is a LAGLIDADG homing endonuclease that initiates mating-type interconversion in yeast. Ho is encoded by a free-standing gene but shows 50% primary sequence similarity to the intein (protein-intron encoded) PI-SceI. Ho is unique among LAGLIDADG endonucleases in having a 120-residue C-terminal putative zinc finger domain. The crystal structure of PI-SceI revealed a bipartite enzyme with a protein-splicing domain (Hint) and intervening endonuclease domain. We made a homology model for Ho on the basis of the PI-SceI structure and performed mutational analysis of putative critical residues, using a mating-type switch as a bioassay for activity and GFP-fusion proteins to detect nuclear localization. We found that residues of the N-terminal sequence of the Hint domain are important for Ho activity, in particular the DNA recognition region. C-terminal residues of the Hint domain are dispensable for Ho activity; however, the C-terminal putative zinc finger domain is essential. Mutational analysis indicated that residues in Ho that are conserved relative to catalytic, active-site residues in PI-SceI and other related homing endonucleases are essential for Ho activity. Our results indicate that in addition to the conserved catalytic residues, Hint domain residues and the zinc finger domain have evolved a critical role in Ho activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.