ArticleGenetics2004
A synthetic lethal screen identifies a role for the cortical actin patch/endocytosis complex in the response to nutrient deprivation in Saccharomyces cerevisiae.
Article in Genetics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 39 citations in OpenAlex.
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- A novel plasmid-based experimental system in Saccharomyces cerevisiae that enables the introduction of 10 different plasmids into cells.FEBS open bio · 2024Article
- Review
- The intersection between stress responses and inositol pyrophosphates in Saccharomyces cerevisiae.Current genetics · 2020Review
- The InsPThe Journal of biological chemistry · 2020Article
- Molecular Architecture of the Inositol Phosphatase Siw14.Biochemistry · 2019Article
- WHI-2 Regulates Intercellular Communication via a MAP Kinase Signaling Complex.Frontiers in microbiology · 2019Article
- Global rewiring of cellular metabolism renders Saccharomyces cerevisiae Crabtree negative.Nature communications · 2018Article
- Structural and biochemical characterization of Siw14: A protein-tyrosine phosphatase fold that metabolizes inositol pyrophosphates.The Journal of biological chemistry · 2018Article
- Bioavailability of Nutritional Resources From Cells Killed by Oxidation Supports Expansion of Survivors inFrontiers in microbiology · 2018Article
- Central roles of iron in the regulation of oxidative stress in the yeast Saccharomyces cerevisiae.Current genetics · 2017Article
- Phosphoribosyl Diphosphate (PRPP): Biosynthesis, Enzymology, Utilization, and Metabolic Significance.Microbiology and molecular biology reviews : MMBR · 2017Review
- A Novel Inositol Pyrophosphate Phosphatase in Saccharomyces cerevisiae: Siw14 PROTEIN SELECTIVELY CLEAVES THE β-PHOSPHATE FROM 5-DIPHOSPHOINOSITOL PENTAKISPHOSPHATE (5PP-IP5).The Journal of biological chemistry · 2016Article
- Histone H2B monoubiquitination is involved in regulating the dynamics of microtubules during the defense response to Verticillium dahliae toxins in Arabidopsis.Plant physiology · 2014Article
- Global analysis of serine/threonine and tyrosine protein phosphatase catalytic subunit genes in Neurospora crassa reveals interplay between phosphatases and the p38 mitogen-activated protein kinase.G3 (Bethesda, Md.) · 2014Article
- Polygenic cis-regulatory adaptation in the evolution of yeast pathogenicity.Genome research · 2012Article
- OsPFA-DSP1, a rice protein tyrosine phosphatase, negatively regulates drought stress responses in transgenic tobacco and rice plants.Plant cell reports · 2012Article
- Phylogenetic and genetic linkage between novel atypical dual-specificity phosphatases from non-metazoan organisms.Molecular genetics and genomics : MGG · 2011Article
- Homoserine toxicity in Saccharomyces cerevisiae and Candida albicans homoserine kinase (thr1Delta) mutants.Eukaryotic cell · 2010Article
- Fungal homoserine kinase (thr1Delta) mutants are attenuated in virulence and die rapidly upon threonine starvation and serum incubation.Eukaryotic cell · 2010Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Saccharomyces cerevisiae whi2Delta cells are unable to halt cell division in response to nutrient limitation and are sensitive to a wide variety of stresses. A synthetic lethal screen resulted in the isolation of siw mutants that had a phenotype similar to that of whi2Delta. Among these were mutations affecting SIW14, FEN2, SLT2, and THR4. Fluid-phase endocytosis is severely reduced or abolished in whi2Delta, siw14Delta, fen2Delta, and thr4Delta mutants. Furthermore, whi2Delta and siw14Delta mutants produce large actin clumps in stationary phase similar to those seen in prk1Delta ark1Delta mutants defective in protein kinases that regulate the actin cytoskeleton. Overexpression of SIW14 in a prk1Delta strain resulted in a loss of cortical actin patches and cables and was lethal. Overexpression of SIW14 also rescued the caffeine sensitivity of the slt2 mutant isolated in the screen, but this was not due to alteration of the phosphorylation state of Slt2. These observations suggest that endocytosis and the organization of the actin cytoskeleton are required for the proper response to nutrient limitation. This hypothesis is supported by the observation that rvs161Delta, sla1Delta, sla2Delta, vrp1Delta, ypt51Delta, ypt52Delta, and end3Delta mutations, which disrupt the organization of the actin cytoskeleton and/or reduce endocytosis, have a phenotype similar to that of whi2Delta mutants.
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