ArticleBMC cell biology2004
Protein-trap version 2.1: screening for expressed proteins in mammalian cells based on their localizations.
Article in BMC cell biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- Protein trap: a new Swiss army knife for geneticists?Molecular biology reports · 2020Review
- Exploring strategies for protein trapping in Drosophila.Genetics · 2007Article
- Improved tagging strategy for protein identification in mammalian cells.BMC genomics · 2005Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background"Protein-trap" is a method that allows epitope-tagging of endogenous proteins. This method allows for the identification of endogenously expressed proteins that exhibit specific localization of interest. This method has been recently reported for its application in the study of Drosophila development by using a relatively large epitope, green-fluorescent-protein (GFP).
resultHerein, we report a new "protein-trap" vector for mammalian cells. This new method utilizes a much smaller epitope-tag and also allows for drug-selection prior to the epitope-tagging. Pre-selection by drug resulted in the highly efficient protein-trapping frequency.
conclusionThe "protein-trap" method based on this new vector is expected to serve as a complimentary approach to the previously reported GFP-based method.
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Registered trials
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