ArticleBMC evolutionary biology2004
Acetylcholinesterase alterations reveal the fitness cost of mutations conferring insecticide resistance.
Article in BMC evolutionary biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 93 citations in OpenAlex.
- Beneficial reversal of dominance maintains a large-effect resistance polymorphism under fluctuating insecticide selection.Nature ecology & evolution · 2025Article
- Enrichment of Hard Sweeps on the X Chromosome in Drosophila melanogaster.Molecular biology and evolution · 2023Article
- Discovery of malathion resistance QTL in Drosophila melanogaster using a bulked phenotyping approach.G3 (Bethesda, Md.) · 2022Article
- Dominance shifts increase the likelihood of soft selective sweeps.Evolution; international journal of organic evolution · 2022Article
- Article
- Developmental changes in larvae of the oriental latrine fly, Chrysomya megacephala, exposed to deltamethrin.Parasitology research · 2021Article
- Fitness effects for Ace insecticide resistance mutations are determined by ambient temperature.BMC biology · 2020Article
- Article
- The relative contribution of target-site mutations in complex acaricide resistant phenotypes as assessed by marker assisted backcrossing in Tetranychus urticae.Scientific reports · 2017Article
- Soft Selective Sweeps in Evolutionary Rescue.Genetics · 2017Article
- Article
- Synergistic and compensatory effects of two point mutations conferring target-site resistance to fipronil in the insect GABA receptor RDL.Scientific reports · 2016Article
- Pyrethroid resistance in Phytoseiulus macropilis (Acari: Phytoseiidae): cross-resistance, stability and effect of synergists.Experimental & applied acarology · 2016Article
- Phe362Tyr in AChE: A Major Factor Responsible for Azamethiphos Resistance in Lepeophtheirus salmonis in Norway.PloS one · 2016Article
- Mechanism behind Resistance against the Organophosphate Azamethiphos in Salmon Lice (Lepeophtheirus salmonis).PloS one · 2015Article
- Article
- Cloning and characterization of prophenoloxidase A3 (proPOA3) from Culex pipiens pallens.Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology · 2012Article
- Cloning and characterization of ribosomal protein S29, a deltamethrin resistance associated gene from Culex pipiens pallens.Parasitology research · 2011Article
- Differential expression of salivary proteins between susceptible and insecticide-resistant mosquitoes of Culex quinquefasciatus.PloS one · 2011Article
- Evidence that adaptation in Drosophila is not limited by mutation at single sites.PLoS genetics · 2010Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundInsecticide resistance is now common in insects due to the frequent use of chemicals to control them, which provides a useful tool to study the adaptation of eukaryotic genome to new environments. Although numerous potential mutations may provide high level of resistance, only few alleles are found in insect natural populations. Then, we hypothesized that only alleles linked to the highest fitness in the absence of insecticide are selected.
resultsTo obtain information on the origin of the fitness of resistant alleles, we studied Drosophila melanogaster acetylcholinesterase, the target of organophosphate and carbamate insecticides. We produced in vitro 15 possible proteins resulting from the combination of the four most frequent mutations and we tested their catalytic activity and enzymatic stability. Mutations affected deacetylation of the enzyme, decreasing or increasing its catalytic efficiency and all mutations diminished the stability of the enzyme. Combination of mutations result to an additive alteration.
conclusionOur findings suggest that the alteration of activity and stability of acetylcholinesterase are at the origin of the fitness cost associated with mutations providing resistance. Magnitude of the alterations was related to the allelic frequency in Drosophila populations suggesting that the fitness cost is the main driving force for the maintenance of resistant alleles in insecticide free conditions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.