ArticleThe Biochemical journal2004
Regulation of expression of the stress response gene, Osp94: identification of the tonicity response element and intracellular signalling pathways.
Article in The Biochemical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 47 citations in OpenAlex.
- Genome-wide characterization and expression analysis of TOPP-type protein phosphatases in soybean (Glycine max L.) reveal the role of GmTOPP13 in drought tolerance.Genes & genomics · 2021Article
- Berberine protects the liver and kidney against functional disorders and histological damages induced by ferrous sulfate.Iranian journal of basic medical sciences · 2018Article
- Quillaja saponaria bark saponin protects Wistar rats against ferrous sulphate-induced oxidative and inflammatory liver damage.Pharmaceutical biology · 2017Article
- Heat shock proteins in the kidney.Pediatric nephrology (Berlin, Germany) · 2016Review
- TonEBP modulates the protective effect of taurine in ischemia-induced cytotoxicity in cardiomyocytes.Cell death & disease · 2015Article
- Protective effects of hesperidin on oxidative stress, dyslipidaemia and histological changes in iron-induced hepatic and renal toxicity in rats.Toxicology reports · 2015Article
- Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress.The Biochemical journal · 2013Article
- NFAT5 in cellular adaptation to hypertonic stress - regulations and functional significance.Journal of molecular signaling · 2013Article
- Transcriptional regulation of gene expression during osmotic stress responses by the mammalian target of rapamycin.Nucleic acids research · 2012Article
- Effects of 2-bromoethanamine on TonEBP expression and its possible role in induction of renal papillary necrosis in mice.Toxicological sciences : an official journal of the Society of Toxicology · 2010Article
- Identification of microRNAs associated with hyperthermia-induced cellular stress response.Cell stress & chaperones · 2010Article
- Molecular cloning of OSP94: A significant biomarker protein of hypertensive human heart and a member of HSP110 family.Molecular biotechnology · 2009Article
- The influence of loud sound stress on expression of osmotic stress protein 94 in the murine inner ear.Neuroscience · 2009Article
- The transcription factor NFAT5 is required for cyclin expression and cell cycle progression in cells exposed to hypertonic stress.PloS one · 2009Article
- Osmotically-induced genes are controlled by the transcription factor TonEBP in cultured cardiomyocytes.Biochemical and biophysical research communications · 2008Article
- Osmoadaptation of Mammalian cells - an orchestrated network of protective genes.Current genomics · 2007Article
- Hyperosmotic stress response: comparison with other cellular stresses.Pflugers Archiv : European journal of physiology · 2007Review
- Hspa4l-deficient mice display increased incidence of male infertility and hydronephrosis development.Molecular and cellular biology · 2006Article
- The protective effect of hydroalcoholic extract of Ginger (Avicenna journal of phytomedicineArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Osp94 (osmotic stress protein of 94 kDa) is known to be up-regulated by hypertonic and heat-shock stresses in mouse renal inner medullary collecting duct (mIMCD3) cells. To investigate the molecular mechanism of transcriptional regulation of the Osp94 gene under these stresses, we cloned and characterized the 5'-flanking region of the gene. Sequence analysis of the proximal 4 kb 5'-flanking region revealed a TATA-less G/C-rich promoter region containing a cluster of Sp1 sites. We also identified upstream sequence motifs similar to the consensus TonE/ORE (tonicity-response element/osmotic response element) as well as the consensus HSE (heat-shock element). Luciferase activities in cells transfected with reporter constructs containing a TonE/ORE-like element (Osp94-TonE; 5'-TGGAAAGGACCAG-3') and HSE enhanced reporter gene expression under hypertonic stress and heat-shock stress respectively. Electrophoretic gel mobility-shift assay showed a slowly migrating band binding to the Osp94-TonE probe, probably representing binding of TonEBP (TonE binding protein) to this enhancer element. Furthermore, treatment of mIMCD3 cells with MAPK (mitogen-activated protein kinase) inhibitors (SB203580, PD98059, U0126 and SP600125) and a proteasome inhibitor (MG132) suppressed the increase in Osp94 gene expression caused by hypertonic NaCl. These results indicate that the 5'-flanking region of Osp94 gene contains a hypertonicity sensitive cis -acting element, Osp94-TonE, which is distinct from a functional HSE. Furthermore, the MAPK and proteasome systems appear to be, at least in part, involved in hypertonic-stressmediated regulation of Osp94 through Osp94-TonE.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.