ArticleThe Biochemical journal2004
RNA interference-mediated silencing of synaptotagmin IX, but not synaptotagmin I, inhibits dense-core vesicle exocytosis in PC12 cells.
Article in The Biochemical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 45 citations in OpenAlex.
- Presynaptic protein Synaptotagmin1 regulates the neuronal polarity and axon differentiation in cultured hippocampal neurons.BMC neuroscience · 2015Article
- Distinct fusion properties of synaptotagmin-1 and synaptotagmin-7 bearing dense core granules.Molecular biology of the cell · 2014Article
- Interaction of anesthetics with neurotransmitter release machinery proteins.Journal of neurophysiology · 2013Article
- Rab33a mediates anterograde vesicular transport for membrane exocytosis and axon outgrowth.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Atg16L1, an essential factor for canonical autophagy, participates in hormone secretion from PC12 cells independently of autophagic activity.Molecular biology of the cell · 2012Article
- Somatodendritic dopamine release requires synaptotagmin 4 and 7 and the participation of voltage-gated calcium channels.The Journal of biological chemistry · 2011Article
- Synaptotagmin 2 couples mucin granule exocytosis to Ca2+ signaling from endoplasmic reticulum.The Journal of biological chemistry · 2009Article
- Genetic analysis of synaptotagmin-7 function in synaptic vesicle exocytosis.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Neuronal calcium sensor-1 and phosphatidylinositol 4-kinase beta stimulate extracellular signal-regulated kinase 1/2 signaling by accelerating recycling through the endocytic recycling compartment.Molecular biology of the cell · 2006Article
- The Slp4-a linker domain controls exocytosis through interaction with Munc18-1.syntaxin-1a complex.Molecular biology of the cell · 2006Article
- Synaptotagmin IV is necessary for the maturation of secretory granules in PC12 cells.The Journal of cell biology · 2006Article
- Anti-sense RNA inhibits the expression of synaptotagmin II in RBL-2H3 and enhances the exocytosis of lysosomes in RBL-2H3.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2005Article
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1 author at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Although PC12 cells express three synaptotagmin isoforms (Syts I, IV and IX), all of which have been proposed to regulate dense-core vesicle exocytosis, it remains unknown which of the Sytisoforms acts as the major Ca2+ sensor for dense-core vesicle exocytosis. In the present study, it has been shown by immunoaffinity purification and immunocytochemistry that Syts I and IX, but not Syt IV, are present on the same secretory vesicles in PC12 cells. Silencing of Syt IX with specific small interfering RNA significantly reduced high KCl-dependent neuropeptide Y secretion from PC12 cells, whereas silencing of Syt I with specific small interfering RNA had no significant effect. The results indicate that Syts I and IX are not functionally equivalent and that Syt IX, and not Syt I, is indispensable for the regulation of Ca2+-dependent dense-core vesicle exocytosis in PC12 cells.
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