ArticleThe EMBO journal2004
Separation of silencing from perinuclear anchoring functions in yeast Ku80, Sir4 and Esc1 proteins.
Article in The EMBO journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 141 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
141 citing papers in PubMed, 263 citations in OpenAlex.
- Telomeres in mitosis: organization and dynamics.Nucleus (Austin, Tex.) · 2026Review
- A translocation within the Ogataea species complex alters local subtelomeric chromatin while maintaining overall genome organization.G3 (Bethesda, Md.) · 2026Article
- Ctf18-dependent localization of interstitial telomeric sequence to nuclear pore complexes prevents chromosome fragility.Nucleic acids research · 2026Article
- A Translocation within thebioRxiv : the preprint server for biology · 2026Article
- RNA Pol I shapes meiotic chromatin, germline H3K4me3 dynamics, and oogenesis independent of ribosome assembly.Cell reports · 2026Article
- Blocking heterochromatin spreading constrains cohesin binding at a yeast heterochromatic locus.PloS one · 2026Article
- RNA Pol I activity is required for meiotic chromatin organization and the H3K4me3 gradient essential for oogenesis, independent of ribosome synthesis.bioRxiv : the preprint server for biology · 2025Article
- Restriction of Ku translocation protects telomere ends.Nature communications · 2025Article
- Review
- The yeast 2-micron plasmid Rep2 protein has Rep1-independent partitioning function.Nucleic acids research · 2022Article
- The regional sequestration of heterochromatin structural proteins is critical to form and maintain silent chromatin.Epigenetics & chromatin · 2022Review
- Review
- Phosphorylation-dependent mitotic SUMOylation drives nuclear envelope-chromatin interactions.The Journal of cell biology · 2021Article
- CRISPR-Cas-Mediated Tethering Recruits the YeastACS synthetic biology · 2021Article
- An overview on current molecular tools for heterologous gene expression in Trichoderma.Fungal biology and biotechnology · 2021Review
- The Multifaceted Roles of Ku70/80.International journal of molecular sciences · 2021Review
- Rad52 SUMOylation functions as a molecular switch that determines a balance between the Rad51- and Rad59-dependent survivors.iScience · 2021Article
- Metabolic regulation of telomere silencing by SESAME complex-catalyzed H3T11 phosphorylation.Nature communications · 2021Article
- Advances in the Relationship Between Regulator of Ribosome Synthesis 1 (RRS1) and Diseases.Frontiers in cell and developmental biology · 2021Review
- The mechanisms and significance of the positional control of centromeres and telomeres in plants.Journal of plant research · 2020Review
81 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In budding yeast, the nuclear periphery forms a subcompartment in which telomeres cluster and SIR proteins concentrate. To identify the proteins that mediate chromatin anchorage to the nuclear envelope, candidates were fused to LexA and targeted to an internal GFP-tagged chromosomal locus. Their ability to shift the locus from a random to a peripheral subnuclear position was monitored in living cells. Using fusions that cannot silence, we identify YKu80 and a 312-aa domain of Sir4 (Sir4(PAD)) as minimal anchoring elements, each able to relocalize an internal chromosomal locus to the nuclear periphery. Sir4(PAD)-mediated tethering requires either the Ku complex or Esc1, an acidic protein that is localized to the inner face of the nuclear envelope even in the absence of Ku, Sir4 or Nup133. Finally, we demonstrate that Ku- and Esc1-dependent pathways mediate natural telomere anchoring in vivo. These data provide the first unambiguous identification of protein interactions that are both necessary and sufficient to localize chromatin to the nuclear envelope.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.