ArticleThe EMBO journal2004
Importin alpha associates with membranes and participates in nuclear envelope assembly in vitro.
Article in The EMBO journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 88 citations in OpenAlex.
- Spindle morphology changes between meiosis and mitosis driven by CK2 regulation of the Ran pathway.The Journal of cell biology · 2025Article
- The localisation and stability of the CENP-F protein are regulated by importin beta and microtubules in mitotic cells.Scientific reports · 2025Article
- Golgi_traff phylogeny reveals ancient eukaryotic genes with recent surprises: replication and diversification of HID1 domain-containing protein unique to Schizosaccharomyces.FEMS microbiology letters · 2025Article
- Normal male fertility in a mouse model of KPNA2 deficiency.PloS one · 2024Article
- Loss of the importiniScience · 2023Article
- KPNA2 promotes angiogenesis by regulating STAT3 phosphorylation.Journal of translational medicine · 2022Article
- SWO1 modulates cell wall integrity under salt stress by interacting with importin ɑ in Arabidopsis.Stress biology · 2021Article
- Membrane association of importin α facilitates viral entry into salivary gland cells of vector insects.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Importin α phosphorylation promotes TPX2 activation by GM130 to control astral microtubules and spindle orientation.Journal of cell science · 2021Article
- Ran pathway-independent regulation of mitotic Golgi disassembly by Importin-α.Nature communications · 2019Article
- Article
- Depletion of nuclear import protein karyopherin alpha 7 (KPNA7) induces mitotic defects and deformation of nuclei in cancer cells.BMC cancer · 2018Article
- Myosin-1C uses a novel phosphoinositide-dependent pathway for nuclear localization.EMBO reports · 2018Article
- Importin α: functions as a nuclear transport factor and beyond.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2018Review
- Neurospora chromosomes are organized by blocks of importin alpha-dependent heterochromatin that are largely independent of H3K9me3.Genome research · 2016Article
- Comparative Proteomic Analysis of Soybean Leaves and Roots by iTRAQ Provides Insights into Response Mechanisms to Short-Term Salt Stress.Frontiers in plant science · 2016Article
- Nuclear transport factors: global regulation of mitosis.Current opinion in cell biology · 2015Review
- GM130 Regulates Golgi-Derived Spindle Assembly by Activating TPX2 and Capturing Microtubules.Cell · 2015Article
- Neurospora importin α is required for normal heterochromatic formation and DNA methylation.PLoS genetics · 2015Article
- Purification of nuclear localization signal-containing proteins and its application to investigation of the mechanisms of the cell division cycle.Small GTPases · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Importin alpha is well known as an adaptor that functions with Importin beta in the nuclear import of proteins containing specific nuclear localization signals (NLSs). We show here that either an excess or a lack of Importin alpha blocks nuclear envelope (NE) assembly in vitro, and our data suggest that soluble Importin alpha functions in NE assembly in conjunction with NLS-containing partner proteins. Surprisingly, a significant proportion of Importin alpha is found to fractionate with Xenopus egg membranes. We demonstrate that membrane association of Importin alpha is regulated by phosphorylation. Using mutant forms of Importin alpha that either do not bind membranes or are not released from them by phosphorylation, we provide evidence that membrane-associated Importin alpha is required for NE formation. Unlike other functions of Importin alpha, this membrane-associated activity does not require interaction with NLS proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.