ArticleThe EMBO journal2004
An intramolecular interaction between the FHA domain and a coiled coil negatively regulates the kinesin motor KIF1A.
Article in The EMBO journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Kinesins inOpen biology · 2025Review
- Comparative analysis of twoeLife · 2024Article
- The architecture of kinesin-3 KLP-6 reveals a multilevel-lockdown mechanism for autoinhibition.Nature communications · 2022Article
- Phosphoregulation of Kinesins Involved in Long-Range Intracellular Transport.Frontiers in cell and developmental biology · 2022Review
- Motor domain-mediated autoinhibition dictates axonal transport by the kinesin UNC-104/KIF1A.PLoS genetics · 2021Article
- Specific KIF1A-adaptor interactions control selective cargo recognition.The Journal of cell biology · 2021Article
- Sequences in the stalk domain regulate auto-inhibition and ciliary tip localization of the immotile kinesin-4 KIF7.Journal of cell science · 2021Article
- A highly conserved 3Science advances · 2021Article
- BCAS2, a protein enriched in advanced prostate cancer, interacts with NBS1 to enhance DNA double-strand break repair.British journal of cancer · 2020Article
- Overexpression ofJournal of cardiovascular development and disease · 2020Article
- An optimized toolbox for the optogenetic control of intracellular transport.The Journal of cell biology · 2020Article
- Intracellular cargo transport by single-headed kinesin motors.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Coiled-coil 1-mediated fastening of the neck and motor domains for kinesin-3 autoinhibition.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- GTP-binding facilitates EB1 recruitment onto microtubules by relieving its auto-inhibition.Scientific reports · 2018Article
- Autosomal dominant transmission of complicated hereditary spastic paraplegia due to a dominant negative mutation of KIF1A, SPG30 gene.Scientific reports · 2017Article
- Autoinhibition of a Neuronal Kinesin UNC-104/KIF1A Regulates the Size and Density of Synapses.Cell reports · 2016Article
- The axonal cytoskeleton: from organization to function.Frontiers in molecular neuroscience · 2015Review
- The family-specific K-loop influences the microtubule on-rate but not the superprocessivity of kinesin-3 motors.Molecular biology of the cell · 2014Article
- The molecular basis for kinesin functional specificity during mitosis.Cytoskeleton (Hoboken, N.J.) · 2013Review
- Neurospora crassa NKIN2, a kinesin-3 motor, transports early endosomes and is required for polarized growth.Eukaryotic cell · 2013Article
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Motor proteins not actively involved in transporting cargoes should remain inactive at sites of cargo loading to save energy and remain available for loading. KIF1A/Unc104 is a monomeric kinesin known to dimerize into a processive motor at high protein concentrations. However, the molecular mechanisms underlying monomer stabilization and monomer-to-dimer transition are not well understood. Here, we report an intramolecular interaction in KIF1A between the forkhead-associated (FHA) domain and a coiled-coil domain (CC2) immediately following the FHA domain. Disrupting this interaction by point mutations in the FHA or CC2 domains leads to a dramatic accumulation of KIF1A in the periphery of living cultured neurons and an enhancement of the microtubule (MT) binding and self-multimerization of KIF1A. In addition, point mutations causing rigidity in the predicted flexible hinge disrupt the intramolecular FHA-CC2 interaction and increase MT binding and peripheral accumulation of KIF1A. These results suggest that the intramolecular FHA-CC2 interaction negatively regulates KIF1A activity by inhibiting MT binding and dimerization of KIF1A, and point to a novel role of the FHA domain in the regulation of kinesin motors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.