ArticleGenes & development2004
Phosphorylation of RCC1 in mitosis is essential for producing a high RanGTP concentration on chromosomes and for spindle assembly in mammalian cells.
Article in Genes & development, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 131 citations in OpenAlex.
- Article
- Unveiling the Movement of RanBP1 During the Cell Cycle and Its Interaction with a Cyclin-Dependent Kinase (CDK) in Plants.International journal of molecular sciences · 2024Article
- Genome-wide identification and expression analysis of theFrontiers in plant science · 2023Article
- Phosphorylation of RCC1 on Serine 11 Facilitates G1/S Transition in HPV E7-Expressing Cells.Biomolecules · 2021Article
- PRMT6 methylation of RCC1 regulates mitosis, tumorigenicity, and radiation response of glioblastoma stem cells.Molecular cell · 2021Article
- RanBP1 controls the Ran pathway in mammalian cells through regulation of mitotic RCC1 dynamics.Cell cycle (Georgetown, Tex.) · 2020Article
- Importin binding mediates the intramolecular regulation of anillin during cytokinesis.Molecular biology of the cell · 2020Article
- The Multifaceted Roles of RCC1 in Tumorigenesis.Frontiers in molecular biosciences · 2020Review
- Phosphorylation of importin-α1 by CDK1-cyclin B1 controls mitotic spindle assembly.Journal of cell science · 2019Article
- Mitotic chromosome binding predicts transcription factor properties in interphase.Nature communications · 2019Article
- Mechanisms that prevent catastrophic interactions between paternal chromosomes and the oocyte meiotic spindle.Cell cycle (Georgetown, Tex.) · 2018Review
- Active Ran regulates anillin function during cytokinesis.Molecular biology of the cell · 2017Article
- Three-dimensional context rather than NLS amino acid sequence determines importin α subtype specificity for RCC1.Nature communications · 2017Article
- Revisiting chromatin binding of the Arabidopsis UV-B photoreceptor UVR8.BMC plant biology · 2016Article
- Non-centrosomal TPX2-Dependent Regulation of the Aurora A Kinase: Functional Implications for Healthy and Pathological Cell Division.Frontiers in oncology · 2016Review
- RanGTP aids anaphase entry through Ubr5-mediated protein turnover.The Journal of cell biology · 2015Article
- Nucleosome functions in spindle assembly and nuclear envelope formation.BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Review
- A NAD-dependent glutamate dehydrogenase coordinates metabolism with cell division in Caulobacter crescentus.The EMBO journal · 2015Article
- Importin-β modulates the permeability of the nuclear pore complex in a Ran-dependent manner.eLife · 2015Article
- Disruption of the ran system by cysteine oxidation of the nucleotide exchange factor RCC1.Molecular and cellular biology · 2015Article
5 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spindle assembly is subject to the regulatory controls of both the cell-cycle machinery and the Ran-signaling pathway. An important question is how the two regulatory pathways communicate with each other to achieve coordinated regulation in mitosis. We show here that Cdc2 kinase phosphorylates the serines located in or near the nuclear localization signal (NLS) of human RCC1, the nucleotide exchange factor for Ran. This phosphorylation is necessary for RCC1 to generate RanGTP on mitotic chromosomes in mammalian cells, which in turn is required for spindle assembly and chromosome segregation. Moreover, phosphorylation of the NLS of RCC1 is required to prevent the binding of importin alpha and beta to RCC1, thereby allowing RCC1 to couple RanGTP production to chromosome binding. These findings reveal that the cell-cycle machinery directly regulates the Ran-signaling pathway by placing a high RanGTP concentration on the mitotic chromosome in mammalian cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.