ArticleProceedings of the National Academy of Sciences of the United States of America2004
Expression profiling with arrays of randomly disrupted genes in mouse embryonic stem cells leads to in vivo functional analysis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Collection of homozygous mutant mouse embryonic stem cells arising from autodiploidization during haploid gene trap mutagenesis.Nucleic acids research · 2018Article
- CIBZ Regulates Mesodermal and Cardiac Differentiation of by Suppressing T and Mesp1 Expression in Mouse Embryonic Stem Cells.Scientific reports · 2016Article
- PiggyBac transposon-based polyadenylation-signal trap for genome-wide mutagenesis in mice.Scientific reports · 2016Article
- The Mammalian Phenotype Ontology as a unifying standard for experimental and high-throughput phenotyping data.Mammalian genome : official journal of the International Mammalian Genome Society · 2012Article
- Evidence that the Upf1-related molecular motor scans the 3'-UTR to ensure mRNA integrity.Nucleic acids research · 2012Article
- Mixture of differentially tagged Tol2 transposons accelerates conditional disruption of a broad spectrum of genes in mouse embryonic stem cells.Nucleic acids research · 2012Article
- CtBP-interacting BTB zinc finger protein (CIBZ) promotes proliferation and G1/S transition in embryonic stem cells via Nanog.The Journal of biological chemistry · 2012Article
- Gene trap mutagenesis in the mouse.Methods in enzymology · 2010Article
- Expression-independent gene trap vectors for random and targeted mutagenesis in embryonic stem cells.Nucleic acids research · 2009Article
- Mutagenesis of diploid mammalian genes by gene entrapment.Nucleic acids research · 2006Article
- Post-entrapment genome engineering: first exon size does not affect the expression of fusion transcripts generated by gene entrapment.Genome research · 2005Article
- Suppression of nonsense-mediated mRNA decay permits unbiased gene trapping in mouse embryonic stem cells.Nucleic acids research · 2005Article
- Assessment of a systematic expression profiling approach in ENU-induced mouse mutant lines.Mammalian genome : official journal of the International Mammalian Genome Society · 2005Article
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Authors and funding
6 authors.
Funding
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Abstract
DNA arrays are capable of profiling the expression patterns of many genes in a single experiment. After finding a gene of interest in a DNA array, however, labor-intensive gene-targeting experiments sometimes must be performed for the in vivo analysis of the gene function. With random gene trapping, on the other hand, it is relatively easy to disrupt and retrieve hundreds of genes/gene candidates in mouse embryonic stem (ES) cells, but one could overlook potentially important gene-disruption events if only the nucleotide sequences and not the expression patterns of the trapped DNA segments are analyzed. To combine the benefits of the above two experimental systems, we first created approximately 900 genetrapped mouse ES cell clones and then constructed arrays of cDNAs derived from the disrupted genes. By using these arrays, we identified a novel gene predominantly expressed in the mouse brain, and the corresponding ES cell clone was used to produced mice homozygous for the disrupted allele of the gene. Detailed analysis of the knockout mice revealed that the gene trap vector completely abolished gene expression downstream of its integration site. Therefore, identification of a gene or novel gene candidate with an interesting expression pattern by using this type of DNA array immediately allows the production of knockout mice from an ES cell clone with a disrupted allele of the sequence of interest.
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