ArticleClinical and experimental immunology2004
Induction of apoptosis in monocytes and lymphocytes by serum from patients with systemic lupus erythematosus - an additional mechanism to increased autoantigen load?
Article in Clinical and experimental immunology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 65 citations in OpenAlex.
- IFN priming is necessary but not sufficient to turn on a migratory dendritic cell program in lupus monocytes.Journal of immunology (Baltimore, Md. : 1950) · 2014Trial
- Tissue macrophages: origin, heterogenity, biological functions, diseases and therapeutic targets.Signal transduction and targeted therapy · 2025Review
- Interleukin-36 upregulates type-I interferon responses in systemic lupus erythematosus by promoting the accumulation of self-nucleic acids.Frontiers in immunology · 2025Article
- Lymphocyte apoptosis and its association with the inflammatory markers and disease severity in juvenile-onset systemic lupus erythematosus patients.Pediatric rheumatology online journal · 2024Article
- CD4 T lymphocyte subsets display heterogeneous susceptibility to apoptosis induced by serum from patients with systemic lupus erythematosus.Advances in rheumatology (London, England) · 2023Article
- The Role of Monocytes and Macrophages in Autoimmune Diseases: A Comprehensive Review.Frontiers in immunology · 2019Review
- Systemic Lupus Erythematosus: Pathogenesis at the Functional Limit of Redox Homeostasis.Oxidative medicine and cellular longevity · 2019Review
- Programmed Cell Death Pathways in the Pathogenesis of Systemic Lupus Erythematosus.Journal of immunology research · 2019Review
- Cell death in the pathogenesis of systemic lupus erythematosus and lupus nephritis.Clinical immunology (Orlando, Fla.) · 2017Review
- Cytokines and MicroRNAs as Candidate Biomarkers for Systemic Lupus Erythematosus.International journal of molecular sciences · 2015Review
- Breaking immunological tolerance in systemic lupus erythematosus.Frontiers in immunology · 2014Review
- The association among leukocyte apoptosis, autoantibodies and disease severity in systemic lupus erythematosus.Journal of translational medicine · 2013Article
- Circulating microRNA expression profiles associated with systemic lupus erythematosus.Arthritis and rheumatism · 2013Article
- Decreased microRNA(miR)-145 and increased miR-224 expression in T cells from patients with systemic lupus erythematosus involved in lupus immunopathogenesis.Clinical and experimental immunology · 2013Article
- Disturbances of apoptotic cell clearance in systemic lupus erythematosus.Arthritis research & therapy · 2011Review
- Monocyte and macrophage abnormalities in systemic lupus erythematosus.Archivum immunologiae et therapiae experimentalis · 2010Review
- Cytokine-induced monocyte characteristics in SLE.Journal of biomedicine & biotechnology · 2010Article
- Complement classical pathway components are all important in clearance of apoptotic and secondary necrotic cells.Clinical and experimental immunology · 2009Article
- Article
- Partial construction of apoptotic pathway in PBMC obtained from active SLE patients and the significance of plasma TNF-alpha on this pathway.Clinical rheumatology · 2006Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The most likely source of autoantigens in systemic lupus erythematosus (SLE) is apoptotic material. Because increased levels of circulating apoptotic cells are found in SLE we wanted to investigate the capacity of serum from patients with SLE or other autoimmune or infectious diseases and normal healthy donors (NHD) to induce apoptosis in normal monocytes, lymphocytes and corresponding cell lines, in relation to clinical and immunological data. Monocytes and lymphocytes from healthy donors were incubated with sera from 37 SLE patients, 37 sex- and age-matched NHD and sera from patients with rheumatoid arthritis, vasculitis, sepsis and mononucleosis. Sera from SLE patients were sampled at both active and inactive disease. The apoptosis-inducing effect (AIE) of these sera was monitored with flow cytometry using annexin V and propidium iodide (PI) binding. The AIE in monocytes and lymphocytes was significantly higher in sera from SLE patients than in other patient groups and NHD (P < 0.001) and was also higher when cell lines were used. Level of C5a in cell culture supernatant correlated with AIE in monocytes (r = 0.451, P = 0.005), suggesting involvement of complement. Heat-inactivation of sera did not affect the AIE, nor did depletion of IgG by protein G absorption of serum. Kinetic analyses showed a peak in apoptosis induction at 12-16 h, with a delayed PI positivity. AIE was equally high using sera from active and inactive SLE cases, and did not correlate with the SLE Disease Activity Index (SLEDAI). Thus, SLE serum has a strong and apparently disease-specific apoptosis-inducing capacity, which could contribute to a high load of potential autoantigen.
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Registered trials
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