ArticleClinical and experimental immunology2004
The effect of eosinophils on collagen gel contraction and implications for tissue remodelling.
Article in Clinical and experimental immunology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 66 citations in OpenAlex.
- Eosinophil biology in COPD: from scientific insight to clinical treatment action.ERJ open research · 2026Review
- Ribonucleases of Mice and Men: Unveiling the Roles of the RNase A Superfamily in Host Defence.Journal of immunology research · 2025Review
- Eosinophils mitigate intestinal fibrosis while promoting inflammation in a chronic DSS colitis model and co-culture model with fibroblasts.Scientific reports · 2024Article
- Understanding fibroblast-immune cell interactionsFrontiers in immunology · 2023Review
- Coro1B and Coro1C regulate lamellipodia dynamics and cell motility by tuning branched actin turnover.The Journal of cell biology · 2022Article
- Innate and adaptive immune system cells implicated in tendon healing and disease.European cells & materials · 2022Review
- Article
- Human RNase3 immune modulation by catalytic-dependent and independent modes in a macrophage-cell line infection model.Cellular and molecular life sciences : CMLS · 2021Article
- Eosinophils and childhood asthma.Clinical and experimental pediatrics · 2021Article
- Investigating Fibroblast-Induced Collagen Gel Contraction Using a Dynamic Microscale Platform.Frontiers in bioengineering and biotechnology · 2019Article
- Activation of Fibroblast Contractility via Cell-Cell Interactions and Soluble Signals.Bio-protocol · 2018Article
- Immune Modulation by Human Secreted RNases at the Extracellular Space.Frontiers in immunology · 2018Review
- Eosinophils in vasculitis: characteristics and roles in pathogenesis.Nature reviews. Rheumatology · 2014Review
- Host protective roles of type 2 immunity: parasite killing and tissue repair, flip sides of the same coin.Seminars in immunology · 2014Review
- A genetic effect of IL-5 receptor α polymorphism in patients with aspirin-exacerbated respiratory disease.Experimental & molecular medicine · 2013Article
- Eosinophils and disease pathogenesis.Seminars in hematology · 2012Review
- Manometric features of eosinophilic esophagitis in esophageal pressure topography.Neurogastroenterology and motility · 2011Article
- Associations of ECP (eosinophil cationic protein)-gene polymorphisms to allergy, asthma, smoke habits and lung function in two Estonian and Swedish sub cohorts of the ECRHS II study.BMC pulmonary medicine · 2010Article
- The effect of PM10 on human lung fibroblasts.Toxicology and industrial health · 2009Article
- Change in inflammation in out-patient COPD patients from stable phase to a subsequent exacerbation.International journal of chronic obstructive pulmonary disease · 2009Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Asthma is characterized by an eosinophilic inflammation and a subepithelial fibrosis in the airways. Eosinophils contain several cytotoxic substances, such as eosinophil cationic protein (ECP), which can promote inflammation and cause tissue damage. This has generated the hypothesis that eosinophils may drive remodelling of extracellular matrix (ECM). To investigate the role of eosinophils we used an in vitro model for remodelling, the three-dimensional collagen gel contraction assay. Two sources of eosinophils were used in this study, isolated human peripheral eosinophils (purity > 95%) and stimulated [interleukin (IL)-5, IL-3 and granulocyte macrophage-colony stimulating factor (GM-CSF)] HL-60 clone 15 cells. Human eosinophils or HL-60 cells were cast together with human lung fibroblasts (HFL1) in type I collagen gels. Both types of eosinophils augmented fibroblast-mediated collagen gel contraction in a time and concentration-dependent manner. At 48 h, the gel area in HFL1/eosinophil co-culture was 46.5% +/- 0.5 (mean +/- s.e.m.) of initial area and in HFL1 culture 52.3% +/- 0.1 (P < 0.001). Respective figures for HFL1/stimulated HL-60 co-culture and HFL1 culture only were 44.1% +/- 0.5 and 52.4% +/- 0.4 (P < 0.001). The release of ECP was increased when fibroblasts were cultured with eosinophils compared to eosinophils cultured alone. In addition, native ECP added to fibroblast gel cultures also augmented contraction. Our results suggest that eosinophils may interact with mesenchymal cells, promoting remodelling of ECM and that ECP constitutes one potential eosinophil-derived mediator driving this process. We conclude that this may be one important mechanism by which eosinophil-ECM interactions will lead to airway tissue remodelling in asthma.
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