ArticleCurrent genetics2004
Isolation and characterization of Saccharomyces cerevisiae mutants with enhanced resistance to the anticancer drug bleomycin.
Article in Current genetics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed, 10 citations in OpenAlex.
- A Universal Stress Protein Involved in Oxidative Stress Is a Phosphorylation Target for Protein Kinase CIPK6.Plant physiology · 2017Article
- Suppression of sensitivity to drugs and antibiotics by high external cation concentrations in fission yeast.PloS one · 2015Article
- Mechanistic studies on bleomycin-mediated DNA damage: multiple binding modes can result in double-stranded DNA cleavage.Nucleic acids research · 2008Article
- Functional genomics of genes with small open reading frames (sORFs) in S. cerevisiae.Genome research · 2006Article
- Characterization of a transport and detoxification pathway for the antitumour drug bleomycin in Saccharomyces cerevisiae.The Biochemical journal · 2004Article
- The uses of genome-wide yeast mutant collections.Genome biology · 2004Review
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bleomycin is an antitumor agent believed to act by damaging DNA. It is currently used for treating testicular carcinomas, but other types of cancers such as ovarian and colon are resistant to the drug from the outset. The mechanism involved in allowing cells to confer resistant to bleomycin is not known. We exploited the power of yeast genetics to isolate for the first time several bleomycin-resistant mutants derived from a strain deleted for the IMP2 gene encoding a transcriptional co-activator. imp2Delta mutants are known to be hypersensitive to bleomycin, monovalent and divalent cations, and high pH. The suppressors of imp2Delta showed extreme resistance to bleomycin and also either fully or partially rescued the phenotypes associated with the imp2Delta mutant, suggesting that bleomycin resistance is linked to other phenotypes. Using fluorescently labeled bleomycin, we demonstrated that two bleomycin-resistant variants, MAY1 and MAY2, were compromised for uptake of the drug, as compared with the parent. In contrast, the imp2Delta mutant showed a substantial increase in the uptake of fluorescently labeled bleomycin. We further showed that strains MAY1 and MAY2 contain a reduced amount of a plasma membrane protein, which binds to (57)Co-labeled bleomycin and is believed to mediate drug entry into the cell. We propose that the bleomycin-resistant mutants are likely defective in a process responsible for transporting the drug into the cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.