ArticleProceedings of the National Academy of Sciences of the United States of America2004
Cholesterol depletion induces PKA-mediated basolateral-to-apical transcytosis of the scavenger receptor class B type I in MDCK cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 71 citations in OpenAlex.
- Cholesterol inhibits assembly and oncogenic activation of the EphA2 receptor.Communications biology · 2025Article
- Cholesterol inhibits assembly and activation of the EphA2 receptor.bioRxiv : the preprint server for biology · 2024Article
- Preparation of chitosan-coated hollow tin dioxide nanoparticles and their application in improving the oral bioavailability of febuxostat.International journal of pharmaceutics: X · 2023Article
- Sonic hedgehog is basolaterally sorted from the TGN and transcytosed to the apical domain involving Dispatched-1 at Rab11-ARE.Frontiers in cell and developmental biology · 2022Article
- Galectin-8 induces partial epithelial-mesenchymal transition with invasive tumorigenic capabilities involving a FAK/EGFR/proteasome pathway in Madin-Darby canine kidney cells.Molecular biology of the cell · 2018Article
- A new model of reverse cholesterol transport: enTICEing strategies to stimulate intestinal cholesterol excretion.Trends in pharmacological sciences · 2015Review
- Differential basolateral-apical distribution of scavenger receptor, class B, type I in cultured cells and the liver.Histochemistry and cell biology · 2014Article
- Intestinal SR-BI does not impact cholesterol absorption or transintestinal cholesterol efflux in mice.Journal of lipid research · 2013Article
- Black pepper and piperine reduce cholesterol uptake and enhance translocation of cholesterol transporter proteins.Journal of natural medicines · 2013Article
- Turnover of C99 is controlled by a crosstalk between ERAD and ubiquitin-independent lysosomal degradation in human neuroglioma cells.PloS one · 2013Article
- N-Glycosylation instead of cholesterol mediates oligomerization and apical sorting of GPI-APs in FRT cells.Molecular biology of the cell · 2011Article
- Adenovirus-mediated sphingomyelin synthase 2 increases atherosclerotic lesions in ApoE KO mice.Lipids in health and disease · 2011Article
- Modulators of Protein Kinase C Affect SR-BI-Dependent HDL Lipid Uptake in Transfected HepG2 Cells.Cholesterol · 2011Article
- Vesicular sterols are essential for synaptic vesicle cycling.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Phosphatidic acid induces ligand-independent epidermal growth factor receptor endocytic traffic through PDE4 activation.Molecular biology of the cell · 2010Article
- Cholesterol-dependent kinase activity regulates transmitter release from cerebellar synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Protein mediators of sterol transport across intestinal brush border membrane.Sub-cellular biochemistry · 2010Review
- Polarized traffic of LRP1 involves AP1B and SNX17 operating on Y-dependent sorting motifs in different pathways.Molecular biology of the cell · 2009Article
- Protein kinase A-dependent step(s) in hepatitis C virus entry and infectivity.Journal of virology · 2008Article
- Membrane trafficking of the human organic anion-transporting polypeptide C (hOATPC).Pharmaceutical research · 2008Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cholesterol-based membrane microdomains, or lipid rafts, are believed to play important, yet poorly defined, roles in protein trafficking and signal transduction. In polarized epithelial cells, the current view is that rafts are involved in apical but not in basolateral protein transport from the trans-Golgi network (TGN). We report here that cholesterol is required in a post-TGN mechanism of basolateral regionalization. Permanently transfected Madin-Darby canine kidney cells segregated the caveolae/raft-associated high-density lipoprotein scavenger receptor class B type I (SR-BI) predominantly to the basolateral domain where it was constitutively internalized and recycled basolaterally. Acute cholesterol depletion did not significantly alter SR-BI internalization, implying a cholesterol depletion-insensitive endocytic process but instead induced its transcytosis through a protein kinase A (PKA)- and microtubule-dependent mechanism. Forskolin also elicited SR-BI transcytosis. The basolateral distribution of endogenous epidermal growth factor receptor remained unaffected. Strikingly, cholesterol depletion induced PKA activity without increasing the cAMP levels. Thus, our results are consistent with a scenario in which cholesterol-based rafts promote internalization and basolateral recycling of internalized SR-BI whereas a PKA pool sensitive to cholesterol depletion mediates SR-BI transcytosis. Regulated transcytosis of SR-BI may provide an additional mechanism to control cholesterol homeostasis. These results disclose relationships between cholesterol-based rafts and PKA activity operating in a post-TGN mechanism of regulated apical-to-basolateral cell surface protein distribution.
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