ArticleNucleic acids research2004
Selenium derivatization and crystallization of DNA and RNA oligonucleotides for X-ray crystallography using multiple anomalous dispersion.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 65 citations in OpenAlex.
- The Unpaved Road of Non-Coding RNA Structure-Function Relationships: Current Knowledge, Available Methodologies, and Future Trends.Non-coding RNA · 2025Review
- Heavy Chalcogen Properties of Sulfur and Selenium Enhance Nucleic Acid-Based Therapeutics.Biomolecules · 2025Review
- Tellurium-Modified Nucleosides, Nucleotides, and Nucleic Acids with Potential Applications.Molecules (Basel, Switzerland) · 2022Review
- Synthesis of DNA and RNA Oligonucleotides Containing a Dual-Purpose Selenium-Modified Fluorescent Nucleoside Probe.Current protocols in nucleic acid chemistry · 2020Article
- Soaking Hexammine Cations into RNA Crystals to Obtain Derivatives for Phasing Diffraction Data.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Independent generation and reactivity of uridin-2'-yl radical.The Journal of organic chemistry · 2014Article
- Structure-based DNA-targeting strategies with small molecule ligands for drug discovery.Medicinal research reviews · 2013Review
- Synthesis of the tellurium-derivatized phosphoramidites and their incorporation into DNA oligonucleotides.Current protocols in nucleic acid chemistry · 2011Article
- Crystal structure of an RNA polymerase ribozyme in complex with an antibody fragment.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2011Article
- Mild detritylation of nucleic acid hydroxyl groups by warming up.Nucleosides, nucleotides & nucleic acids · 2011Article
- A portable RNA sequence whose recognition by a synthetic antibody facilitates structural determination.Nature structural & molecular biology · 2011Article
- Phosphoramidates of 2'-beta-D-arabinouridine (AraU) as phosphate prodrugs; design, synthesis, in vitro activity and metabolism.Bioorganic & medicinal chemistry · 2010Article
- A fast selenium derivatization strategy for crystallization and phasing of RNA structures.RNA (New York, N.Y.) · 2009Article
- Determining structures of RNA aptamers and riboswitches by X-ray crystallography.Methods in molecular biology (Clifton, N.J.) · 2009Article
- Selenium derivatization of nucleic acids for phase and structure determination in nucleic acid X-ray crystallography.International journal of molecular sciences · 2008Article
- A general strategy to solve the phase problem in RNA crystallography.Structure (London, England : 1993) · 2007Article
- Synthesis of a 2'-Se-thymidine phosphoramidite and its incorporation into oligonucleotides for crystal structure study.Organic letters · 2007Article
- Selenium derivatization of nucleic acids for crystallography.Nucleic acids research · 2007Article
- Efficient substrate cleavage catalyzed by hammerhead ribozymes derivatized with selenium for X-ray crystallography.Biochemistry · 2006Article
- Synthesis of a 2'-Se-uridine phosphoramidite and its incorporation into oligonucleotides for structural study.Organic letters · 2005Article
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5 authors at 1 institution in 1 country.
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Abstract
We report here the solid phase synthesis of RNA and DNA oligonucleotides containing the 2'-selenium functionality for X-ray crystallography using multiwavelength anomalous dispersion. We have synthesized the novel 2'-methylseleno cytidine phosphoramidite and improved the accessibility of the 2'-methylseleno uridine phosphoramidite for the synthesis of many selenium-derivatized DNAs and RNAs in large scales. The yields of coupling these Se-nucleoside phosphoramidites into DNA or RNA oligonucleotides were over 99% when 5-(benzylmercapto)-1H-tetrazole was used as the coupling reagent. The UV melting study of A-form dsDNAs indicated that the 2'-selenium derivatization had no effect on the stability of the duplexes with the 3'-endo sugar pucker. Thus, the stems of functional RNA molecules with the same 3'-endo sugar pucker appear to be the ideal sites for the selenium derivatization with 2'-Se-C and 2'-Se-U. Crystallization of the selenium-derivatized oligonucleotides is also reported here. The results demonstrate that this 2'-selenium functionality is suitable for RNA and A-form DNA derivatization in X-ray crystallography.
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