ArticleApplied and environmental microbiology2004
Rapid detection and enumeration of Legionella pneumophila in hot water systems by solid-phase cytometry.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed.
- Determination of viable legionellae in engineered water systems: Do we find what we are looking for?Water research · 2016Review
- Fast immunosensing technique to detect Legionella pneumophila in different natural and anthropogenic environments: comparative and collaborative trials.BMC microbiology · 2013Article
- Rapid detection of total and viable Legionella pneumophila in tap water by immunomagnetic separation, double fluorescent staining and flow cytometry.Microbial biotechnology · 2012Article
- Rapid and sensitive quantification of Vibrio cholerae and Vibrio mimicus cells in water samples by use of catalyzed reporter deposition fluorescence in situ hybridization combined with solid-phase cytometry.Applied and environmental microbiology · 2012Article
- Total and viable Legionella pneumophila cells in hot and natural waters as measured by immunofluorescence-based assays and solid-phase cytometry.Applied and environmental microbiology · 2011Article
- High diversity and abundance of Legionella spp. in a pristine river and impact of seasonal and anthropogenic effects.Applied and environmental microbiology · 2010Article
- Rapid and direct quantification of viable Candida species in whole blood by use of immunomagnetic separation and solid-phase cytometry.Journal of clinical microbiology · 2010Article
- Detection and quantification of viable airborne bacteria and fungi using solid-phase cytometry.Nature protocols · 2009Article
- Occurrence and genetic diversity of uncultured Legionella spp. in drinking water treated at temperatures below 15 degrees C.Applied and environmental microbiology · 2006Article
- Rapid method for enumeration of viable Legionella pneumophila and other Legionella spp. in water.Applied and environmental microbiology · 2005Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new method for the rapid and sensitive detection of Legionella pneumophila in hot water systems has been developed. The method is based on an IF assay combined with detection by solid-phase cytometry. This method allowed the enumeration of L. pneumophila serogroup 1 and L. pneumophila serogroups 2 to 6, 8 to 10, and 12 to 15 in tap water samples within 3 to 4 h. The sensitivity of the method was between 10 and 100 bacteria per liter and was principally limited by the filtration capacity of membranes. The specificity of the antibody was evaluated against 15 non-Legionella strains, and no cross-reactivity was observed. When the method was applied to natural waters, direct counts of L. pneumophila were compared with the number of CFU obtained by the standard culture method. Direct counts were always higher than culturable counts, and the ratio between the two methods ranged from 1.4 to 325. Solid-phase cytometry offers a fast and sensitive alternative to the culture method for L. pneumophila screening in hot water systems.
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Registered trials
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