ArticleApplied and environmental microbiology2004
Influence of lipoteichoic acid D-alanylation on protein secretion in Lactococcus lactis as revealed by random mutagenesis.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- Occurrence and potential mechanism of holin-mediated non-lytic protein translocation in bacteria.Microbial cell (Graz, Austria) · 2022Review
- Using Knock-Out Mutants to Investigate the Adhesion ofInternational journal of molecular sciences · 2021Article
- Spatial regulation of protein A in Staphylococcus aureus.Molecular microbiology · 2021Article
- Cell wall structure and function in lactic acid bacteria.Microbial cell factories · 2014Review
- Inactivation of the ybdD gene in Lactococcus lactis increases the amounts of exported proteins.Applied and environmental microbiology · 2012Article
- N-acetylglucosamine-6-phosphate deacetylase (NagA) of Listeria monocytogenes EGD, an essential enzyme for the metabolism and recycling of amino sugars.Archives of microbiology · 2012Article
- Genetic analysis of SCO2997, encoding a TagF homologue, indicates a role for wall teichoic acids in sporulation of Streptomyces coelicolor A3(2).Journal of bacteriology · 2011Article
- Role of the cell wall microenvironment in expression of a heterologous SpaP-S1 fusion protein by Streptococcus gordonii.Applied and environmental microbiology · 2011Article
- Assessment of the diversity of dairy Lactococcus lactis subsp. lactis isolates by an integrated approach combining phenotypic, genomic, and transcriptomic analyses.Applied and environmental microbiology · 2011Article
- Distinct and essential morphogenic functions for wall- and lipo-teichoic acids in Bacillus subtilis.The EMBO journal · 2009Article
- Inactivation of DltA modulates virulence factor expression in Streptococcus pyogenes.PloS one · 2009Article
- Random mutagenesis identifies novel genes involved in the secretion of antimicrobial, cell wall-lytic enzymes by Lactococcus lactis.Applied and environmental microbiology · 2008Article
- Variations in the degree of D-Alanylation of teichoic acids in Lactococcus lactis alter resistance to cationic antimicrobials but have no effect on bacterial surface hydrophobicity and charge.Applied and environmental microbiology · 2008Article
- Localization and interactions of teichoic acid synthetic enzymes in Bacillus subtilis.Journal of bacteriology · 2008Article
- A functional dlt operon, encoding proteins required for incorporation of d-alanine in teichoic acids in gram-positive bacteria, confers resistance to cationic antimicrobial peptides in Streptococcus pneumoniae.Journal of bacteriology · 2006Article
- Complementation of the Lactococcus lactis secretion machinery with Bacillus subtilis SecDF improves secretion of staphylococcal nuclease.Applied and environmental microbiology · 2006Article
- Protein secretion in Lactococcus lactis : an efficient way to increase the overall heterologous protein production.Microbial cell factories · 2005Article
- Autolysis of Lactococcus lactis is increased upon D-alanine depletion of peptidoglycan and lipoteichoic acids.Journal of bacteriology · 2005Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lactococcus lactis, a food-grade nonpathogenic lactic acid bacterium, is a good candidate for the production of heterologous proteins of therapeutic interest. We examined host factors that affect secretion of heterologous proteins in L. lactis. Random insertional mutagenesis was performed with L. lactis strain MG1363 carrying a staphylococcal nuclease (Nuc) reporter cassette in its chromosome. This cassette encodes a fusion protein between the signal peptide of the Usp45 lactococcal protein and the mature moiety of a truncated form of Nuc (NucT). The Nuc secretion efficiency (secreted NucT versus total NucT) from this construct is low in L. lactis (approximately 40%). Twenty mutants affected in NucT production and/or in secretion capacity were selected and identified. In these mutants, several independent insertions mapped in the dltA gene (involved in D-alanine transfer in lipoteichoic acids) and resulted in a NucT secretion defect. Characterization of the dltA mutant phenotype with respect to NucT secretion revealed that it is involved in a late secretion stage by causing mature NucT entrapment at the cell surface.
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