ArticleApplied and environmental microbiology2004
Construction of a functional S-layer fusion protein comprising an immunoglobulin G-binding domain for development of specific adsorbents for extracorporeal blood purification.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 109 citations in OpenAlex.
- Scalable biomimetic sensing system with membrane receptor dual-monolayer probe and graphene transistor arrays.Science advances · 2023Article
- A New Method for Dispersing Pristine Carbon Nanotubes Using Regularly Arranged S-Layer Proteins.Nanomaterials (Basel, Switzerland) · 2021Article
- Electrochemical Biosensors Based on S-Layer Proteins.Sensors (Basel, Switzerland) · 2020Review
- Structural basis of cell wall anchoring by SLH domains in Paenibacillus alvei.Nature communications · 2018Article
- S-Layer Protein-Based Biosensors.Biosensors · 2018Article
- In Vitro Characterization of the Two-Stage Non-Classical Reassembly Pathway of S-Layers.International journal of molecular sciences · 2017Article
- Targeting at the Nanoscale: A Novel S-Layer Fusion Protein Enabling Controlled Immobilization of Biotinylated Molecules.Nanomaterials (Basel, Switzerland) · 2016Article
- S-layer fusion protein as a tool functionalizing emulsomes and CurcuEmulsomes for antibody binding and targeting.Colloids and surfaces. B, Biointerfaces · 2015Article
- Emulsomes meet S-layer proteins: an emerging targeted drug delivery system.Current pharmaceutical biotechnology · 2015Review
- S-layers: principles and applications.FEMS microbiology reviews · 2014Article
- S-layer protein self-assembly.International journal of molecular sciences · 2013Article
- Relevant uses of surface proteins--display on self-organized biological structures.Microbial biotechnology · 2012Review
- S-layer fusion proteins--construction principles and applications.Current opinion in biotechnology · 2011Review
- Heterologous protein display on the cell surface of lactic acid bacteria mediated by the s-layer protein.Microbial cell factories · 2011Article
- Fast and easy protocol for the purification of recombinant S-layer protein for synthetic biology applications.Biotechnology journal · 2011Article
- Exploitation of the S-layer self-assembly system for site directed immobilization of enzymes demonstrated for an extremophilic laminarinase from Pyrococcus furiosus.Journal of biotechnology · 2008Article
- S-layer-mediated display of the immunoglobulin G-binding domain of streptococcal protein G on the surface of Caulobacter crescentus: development of an immunoactive reagent.Applied and environmental microbiology · 2007Article
- Immunoabsorbent nanoparticles based on a tobamovirus displaying protein A.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Structure, surface interactions, and compressibility of bacterial S-layers through scanning force microscopy and the surface force apparatus.Biophysical journal · 2006Article
- Recent advances of protein microarrays.Current opinion in chemical biology · 2006Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The chimeric gene encoding a C-terminally-truncated form of the S-layer protein SbpA from Bacillus sphaericus CCM 2177 and two copies of the Fc-binding Z-domain was constructed, cloned, and heterologously expressed in Escherichia coli HMS174(DE3). The Z-domain is a synthetic analogue of the B-domain of protein A, capable of binding the Fc part of immunoglobulin G (IgG). The S-layer fusion protein rSbpA(31-1068)/ZZ retained the specific properties of the S-layer protein moiety to self-assemble in suspension and to recrystallize on supports precoated with secondary cell wall polymer (SCWP), which is the natural anchoring molecule for the S-layer protein in the bacterial cell wall. Due to the construction principle of the S-layer fusion protein, the ZZ-domains remained exposed on the outermost surface of the protein lattice. The binding capacity of the native or cross-linked monolayer for human IgG was determined by surface plasmon resonance measurements. For batch adsorption experiments, 3-microm-diameter, biocompatible cellulose-based, SCWP-coated microbeads were used for recrystallization of the S-layer fusion protein. In the case of the native monolayer, the binding capacity for human IgG was 5.1 ng/mm(2), whereas after cross-linking with dimethyl pimelimidate, 4.4 ng of IgG/mm(2) was bound. This corresponded to 78 and 65% of the theoretical saturation capacity of a planar surface for IgGs aligned in the upright position, respectively. Compared to commercial particles used as immunoadsorbents to remove autoantibodies from sera of patients suffering from an autoimmune disease, the IgG binding capacity of the S-layer fusion protein-coated microbeads was at least 20 times higher. For that reason, this novel type of microbeads should find application in the microsphere-based detoxification system.
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