ArticleApplied and environmental microbiology2004
Simultaneous detection and identification of common cell culture contaminant and pathogenic mollicutes strains by reverse line blot hybridization.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Microbiota of the indoor environment: a meta-analysis.Microbiome · 2015Pooled it
- Sterility Testing for Cellular Therapies: What Is the Role of the Clinical Microbiology Laboratory?Journal of clinical microbiology · 2020Review
- Effects of the Brown SeaweedFrontiers in nutrition · 2018Article
- Global investigation of composition and interaction networks in gut microbiomes of individuals belonging to diverse geographies and age-groups.Gut pathogens · 2016Article
- The expression of pluripotency genes and neuronal markers after neurodifferentiation in fibroblasts co-cultured with human umbilical cord blood mononuclear cells.In vitro cellular & developmental biology. Animal · 2015Article
- Development of a PCR assay to detect mycoplasma contamination in cord blood hematopoietic stem cells.Iranian journal of microbiology · 2014Article
- Molecular methods for the detection of Mycoplasma and ureaplasma infections in humans: a paper from the 2011 William Beaumont Hospital Symposium on molecular pathology.The Journal of molecular diagnostics : JMD · 2012Review
- Performance of different mono- and multiplex nucleic acid amplification tests on a multipathogen external quality assessment panel.Journal of clinical microbiology · 2012Article
- Multiplex PCR and reverse line blot hybridization assay (mPCR/RLB).Journal of visualized experiments : JoVE · 2011Article
- Acute respiratory infection due to Mycoplasma pneumoniae: current status of diagnostic methods.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2010Review
- Simultaneous identification of 14 genital microorganisms in urine by use of a multiplex PCR-based reverse line blot assay.Journal of clinical microbiology · 2009Article
- Biological enrichment of Mycoplasma agents by cocultivation with permissive cell cultures.Applied and environmental microbiology · 2008Article
- Simultaneous detection and identification of Candida, Aspergillus, and Cryptococcus species by reverse line blot hybridization.Journal of clinical microbiology · 2006Article
- Simultaneous detection of nine antibiotic resistance-related genes in Streptococcus agalactiae using multiplex PCR and reverse line blot hybridization assay.Antimicrobial agents and chemotherapy · 2006Article
- Reverse line blot assay for direct identification of seven Streptococcus agalactiae major surface protein antigen genes.Clinical and vaccine immunology : CVI · 2006Article
- Tandem use of immunofluorescent and DNA staining assays to validate nested PCR detection of mycoplasma.In vitro cellular & developmental biology. AnimalArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a reverse line blot (RLB) hybridization assay to detect and identify the commonest mollicutes causing cell line contamination (Mycoplasma arginini, Mycoplasma fermentans, Mycoplasma hyorhinis, Mycoplasma orale, and Acholeplasma laidlawii) and human infection (Mycoplasma pneumoniae, Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma parvum, and Ureaplasma urealyticum). We developed a nested PCR assay with "universal" primers targeting the mollicute 16S-23S rRNA intergenic spacer region. Amplified biotin-labeled PCR products were hybridized to membrane-bound species-specific oligonucleotide probes. The assay correctly identified reference strains of 10 mollicute species. Cell cultures submitted for detection of mollicute contamination, clinical specimens, and clinical isolates were initially tested by PCR assay targeting a presumed mollicute-specific sequence of the 16S rRNA gene. Any that were positive were assessed by the RLB assay, with species-specific PCR assay as the reference method. Initially, 100 clinical and 88 of 92 cell culture specimens gave concordant results, including 18 in which two or more mollicute species were detected by both methods. PCR and sequencing of the 16S-23S rRNA intergenic spacer region and subsequent retesting by species-specific PCR assay of the four cell culture specimens for which results were initially discrepant confirmed the original RLB results. Sequencing of amplicons from 12 cell culture specimens that were positive in the 16S rRNA PCR assay but negative by both the RLB and species-specific PCR assays failed to identify any mollicute species. The RLB hybridization assay is sensitive and specific and able to rapidly detect and identify mollicute species from clinical and cell line specimens.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.