ArticleThe Journal of cell biology1992
2-Deoxyglucose and cytochalasin D modulate aldolase mobility in living 3T3 cells.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 71 citations in OpenAlex.
- Mechanisms of actin-binding proteins in glycolysis-cytoskeleton coupling.Journal of translational medicine · 2026Review
- Expression of Genes in the 16p11.2 Locus during Development of the Human Fetal Cerebral Cortex.Cerebral cortex (New York, N.Y. : 1991) · 2021Article
- Fructose-Bisphosphate Aldolase A Regulates Hypoxic Adaptation in Hepatocellular Carcinoma and Involved with Tumor Malignancy.Digestive diseases and sciences · 2019Article
- Article
- Targeting of several glycolytic enzymes using RNA interference reveals aldolase affects cancer cell proliferation through a non-glycolytic mechanism.The Journal of biological chemistry · 2012Article
- Glutamate dehydrogenase in brain mitochondria: do lipid modifications and transient metabolon formation influence enzyme activity?Neurochemistry international · 2011Review
- MIC6 associates with aldolase in host cell invasion by Toxoplasma gondii.Parasitology research · 2009Article
- Pregnancy alters glucose-6-phosphate dehydrogenase trafficking, cell metabolism, and oxidant release of maternal neutrophils.The Journal of clinical investigation · 2002Article
- Localization of GAR transformylase in Escherichia coli and mammalian cells.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Acute regulation of glucose transport in a monocyte-macrophage cell line: Glut-3 affinity for glucose is enhanced during the respiratory burst.The Biochemical journal · 1997Article
- 19F NMR measurements of the rotational mobility of proteins in vivo.Biophysical journal · 1997Article
- The dynamics of local kinetic parameters of glutamate dehydrogenase in rat liver.Histochemistry and cell biology · 1996Article
- Substrate modulation of aldolase B binding in hepatocytes.The Biochemical journal · 1996Article
- Analysis of enzyme reactions in situ.The Histochemical journal · 1995Review
- Open reading frames in the antisense strands of genes coding for glycolytic enzymes in Saccharomyces cerevisiae.Molecular & general genetics : MGG · 1994Article
- Direct observation of hexokinase translocation in stimulated macrophages.The Biochemical journal · 1993Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Approximately 23% of the glycolytic enzyme aldolase in the perinuclear region of Swiss 3T3 cells is immobile as measured by FRAP. Previous studies suggest that the immobile fraction may be associated with the actin cytoskeleton (Pagliaro, L. and D. L. Taylor. 1988. J. Cell Biol. 107:981-991), and it has been proposed that the association of some glycolytic enzymes with the cytoskeleton could have functional significance, perhaps involving a fundamental relationship between glycolysis, cytoplasmic organization, and cell motility. We have tested the effect of a key glycolytic inhibitor and an actin cytoskeletal modulator on the mobility of aldolase in living cells directly, using fluorescent analog cytochemistry and FRAP. We report here that the competitive hexokinase inhibitor 2-deoxyglucose releases the bound fraction of aldolase in 3T3 cells within 10 min, and that this process is reversible upon washout of the inhibitor. A similar result is produced with the actin-binding agent, cytochalasin D. These results are consistent with models in which glycolytic enzymes are not exclusively diffusion-limited, soluble proteins, but may exist partially in the solid phase of cytoplasm. Such organization has significant implications for both the modulation of cytoplasmic structure and for cellular metabolism.
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