ArticleNucleic acids research2004
Sequence-specific cleavage of RNA in the absence of divalent metal ions by a DNAzyme incorporating imidazolyl and amino functionalities.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 122 citations in OpenAlex.
- Advances in DNAzyme Selection, Molecular Engineering and Biomedical Applications.International journal of molecular sciences · 2026Review
- Functional Analysis of Histidine-Dependent Self-Cleaving RNAs Obtained by In Vitro Selection.Chembiochem : a European journal of chemical biology · 2025Article
- Polymerase Synthesis of Hypermodified DNA Displaying a Combination of Thiol, Hydroxyl, Carboxylate, and Imidazole Functional Groups in the Major Groove.Chemistry (Weinheim an der Bergstrasse, Germany) · 2025Article
- Zwitterionic DNA: enzymatic synthesis of hypermodified DNA bearing four different cationic substituents at all four nucleobases.Nucleic acids research · 2025Article
- L-Histidine Modulates the Catalytic Activity and Conformational Changes of the HD3 Deoxyribozyme.Genes · 2024Article
- A toolbox for enzymatic modification of nucleic acids with photosensitizers for photodynamic therapy.RSC chemical biology · 2024Article
- In vitro evolution of ribonucleases from expanded genetic alphabets.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- An RNA-cleaving threose nucleic acid enzyme capable of single point mutation discrimination.Nature chemistry · 2022Article
- Nonenzymatic assembly of active chimeric ribozymes from aminoacylated RNA oligonucleotides.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Environmental and intercellular PbMikrochimica acta · 2020Article
- In vitro Selection of Chemically Modified DNAzymes.ChemistryOpen · 2020Review
- Synthetic Life with Alternative Nucleic Acids as Genetic Materials.Molecules (Basel, Switzerland) · 2020Review
- Molecular Features and Metal Ions That Influence 10-23 DNAzyme Activity.Molecules (Basel, Switzerland) · 2020Review
- Site-specific functionalization with amino, guanidinium, and imidazolyl groups enabling the activation of 10-23 DNAzyme.RSC advances · 2020Article
- Modified nucleotides may have enhanced early RNA catalysis.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Side chain determinants of biopolymer function during selection and replication.Nature chemical biology · 2019Article
- Tethered imidazole mediated duplex stabilization and its potential for aptamer stabilization.Nucleic acids research · 2018Article
- Evolution of sequence-defined highly functionalized nucleic acid polymers.Nature chemistry · 2018Article
- A densely modified MChemical science · 2018Article
- Selection of DNA aptamers with two modified bases.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Two modified 2'-deoxynucleoside 5'-triphosphates have been used for the in vitro selection of a modified deoxyribozyme (DNAzyme) capable of the sequence-specific cleavage of a 12 nt RNA target in the absence of divalent metal ions. The modified nucleotides, a C5-imidazolyl-modified dUTP and 3-(aminopropynyl)-7-deaza-dATP were used in place of TTP and dATP during the selection and incorporate two extra protein-like functionalities, namely, imidazolyl (histidine analogue) and primary amino (lysine analogue) into the DNAzyme. The functional groups are analogous to the catalytic Lys and His residues employed during the metal-independent cleavage of RNA by the protein enzyme RNaseA. The DNAzyme requires no divalent metal ions or other cofactors for catalysis, remains active at physiological pH and ionic strength and can recognize and cleave a 12 nt RNA substrate with sequence specificity. This is the first example of a functionalized, metal-independent DNAzyme that recognizes and cleaves an all-RNA target in a sequence-specific manner. The selected DNAzyme is two orders of magnitude more efficient in its cleavage of RNA than an unmodified DNAzyme in the absence of metal ions and represents a rate enhancement of 10(5) compared with the uncatalysed hydrolysis of RNA.
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