ArticleNucleic acids research2004
Influence of in vitro manipulation on the stability of methylation patterns in the Snurf/Snrpn-imprinting region in mouse embryonic stem cells.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 43 citations in OpenAlex.
- A Link between Genetic Disorders and Cellular Impairment, Using Human Induced Pluripotent Stem Cells to Reveal the Functional Consequences of Copy Number Variations in the Central Nervous System-A Close Look at Chromosome 15.International journal of molecular sciences · 2020Review
- IPSC Models of Chromosome 15Q Imprinting Disorders: From Disease Modeling to Therapeutic Strategies.Advances in neurobiology · 2020Article
- [Methyltransferase inhibitor BIX01294 promotes the migration and inhibits decidualization of mouse uterine stromal cells in vitro].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2017Article
- Effect of BIX-01294 on H3K9me2 levels and the imprinted gene Snrpn in mouse embryonic fibroblast cells.Bioscience reports · 2015Article
- Comparison of reprogramming genes in induced pluripotent stem cells and nuclear transfer cloned embryos.Stem cell reviews and reports · 2014Article
- Haploid genomes illustrate epigenetic constraints and gene dosage effects in mammals.Epigenetics & chromatin · 2013Article
- Zinc finger protein ZFP57 requires its co-factor to recruit DNA methyltransferases and maintains DNA methylation imprint in embryonic stem cells via its transcriptional repression domain.The Journal of biological chemistry · 2012Article
- A high-throughput DNA methylation analysis of a single cell.Nucleic acids research · 2011Article
- Epigenetic regulation of mesenchymal stem cells: a focus on osteogenic and adipogenic differentiation.Stem cells international · 2011Article
- Induced pluripotent stem cell models of the genomic imprinting disorders Angelman and Prader-Willi syndromes.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Addition of H19 'loss of methylation testing' for Beckwith-Wiedemann syndrome (BWS) increases the diagnostic yield.The Journal of molecular diagnostics : JMD · 2010Article
- Germline competence of mouse ES and iPS cell lines: Chimera technologies and genetic background.World journal of stem cells · 2009Article
- Decreased surfactant protein B expression in mice derived completely from embryonic stem cells.In vitro cellular & developmental biology. AnimalArticle
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent work on embryonic stem (ES) cells showed that stem cell-derived tissues and embryos, cloned from ES cell nuclei, often fail to maintain epigenetic states of imprinted genes. This deregulation is frequently associated with in vitro manipulations and culture conditions which might affect the cells potential to develop into normal fetuses. Usually, epigenetic instability is reported in differentially methylated regions of mostly growth-related imprinted genes. However, little is known about the epigenetic stability of genes that function late in organogenesis. Hence, we set out to investigate the epigenetic stability of neuronal genes and analyzed DNA methylation patterns in the Snurf/Snrpn imprinted cluster in several cultured mouse ES cell lines. We also determined the effects of in vitro stress factors such as consecutive passaging, trypsination, mechanical handling, single cell cloning, centrifugation, staurosporine-induced neurogenesis and the insertion of viral (foreign) DNA into the host genome. Intriguingly, none of these in vitro manipulations interfered with the stability of the methylation patterns in the analyzed neuronal genes. These data imply that, in contrast to growth-related genes like Igf2, H19, Igf2r or Grb10, the methylation imprints of the analyzed neuronal genes in the Snurf/Snrpn cluster may be particularly stable in manipulated ES cells.
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