ArticleMolecular biology of the cell2004
Identification of novel principles of keratin filament network turnover in living cells.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed, 95 citations in OpenAlex.
- Desmosomes compartmentalize mRNA and translation in the skin.Developmental cell · 2026Article
- Fast-scanning small-angle X-ray scattering of hydrated biological cells.Journal of synchrotron radiation · 2026Article
- SRC and ERK Regulate the Turnover of Cytoskeletal Keratin Filaments.International journal of molecular sciences · 2025Article
- Candidate proteins interacting with cytoskeleton in cells from the basal airway epitheliumFrontiers in molecular biosciences · 2024Article
- Effects of truncations in the N- and C-terminal domains of filensin on filament formation with phakinin in cell-free conditions and cultured cells.FEBS open bio · 2023Article
- Quantitative mapping of keratin networks in 3D.eLife · 2022Article
- Intermediate filaments: Integration of cell mechanical properties during migration.Frontiers in cell and developmental biology · 2022Review
- Keratins as an Inflammation Trigger Point in Epidermolysis Bullosa Simplex.International journal of molecular sciences · 2021Review
- Structural heterogeneity of cellular K5/K14 filaments as revealed by cryo-electron microscopy.eLife · 2021Article
- Hemidesmosome-Related Keratin Filament Bundling and Nucleation.International journal of molecular sciences · 2021Article
- 14-3-3 targets keratin intermediate filaments to mechanically sensitive cell-cell contacts.Molecular biology of the cell · 2020Article
- Keratin Dynamics and Spatial Distribution in Wild-Type and K14 R125P Mutant Cells-A Computational Model.International journal of molecular sciences · 2020Article
- The keratin-desmosome scaffold: pivotal role of desmosomes for keratin network morphogenesis.Cellular and molecular life sciences : CMLS · 2020Article
- Kinesin-dependent transport of keratin filaments: a unified mechanism for intermediate filament transport.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Types I and II Keratin Intermediate Filaments.Cold Spring Harbor perspectives in biology · 2018Review
- Atomic structures of low-complexity protein segments reveal kinked β sheets that assemble networks.Science (New York, N.Y.) · 2018Article
- Intracellular Motility of Intermediate Filaments.Cold Spring Harbor perspectives in biology · 2017Review
- Intermediate filament dynamics: What we can see now and why it matters.BioEssays : news and reviews in molecular, cellular and developmental biology · 2016Review
- Vimentin filament precursors exchange subunits in an ATP-dependent manner.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Dissection of keratin network formation, turnover and reorganization in living murine embryos.Scientific reports · 2015Article
Corrections and comments
- Commented on by
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is generally assumed that turnover of the keratin filament system occurs by exchange of subunits along its entire length throughout the cytoplasm. We now present evidence that a circumscribed submembranous compartment is actually the main site for network replenishment. This conclusion is based on the following observations in living cells synthesizing fluorescent keratin polypeptides: 1) Small keratin granules originate in close proximity to the plasma membrane and move toward the cell center in a continuous motion while elongating into flexible rod-like fragments that fuse with each other and integrate into the peripheral KF network. 2) Recurrence of fluorescence after photobleaching is first seen in the cell periphery where keratin filaments are born that translocate subsequently as part of the network toward the cell center. 3) Partial keratin network reformation after orthovanadate-induced disruption is restricted to a distinct peripheral zone in which either keratin granules or keratin filaments are transiently formed. These findings extend earlier investigations of mitotic cells in which de novo keratin network formation was shown to originate from the cell cortex. Taken together, our results demonstrate that the keratin filament system is not homogeneous but is organized into temporally and spatially distinct subdomains. Furthermore, the cortical localization of the regulatory cues for keratin filament turnover provides an ideal way to adjust the epithelial cytoskeleton to dynamic cellular processes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.