ArticleMolecular biology of the cell2004
Biophysical parameters influence actin-based movement, trajectory, and initiation in a cell-free system.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 50 citations in OpenAlex.
- Spontaneous membrane protrusion and cell morphogenesis via self-propelled actin filaments.EMBO reports · 2026Article
- The Transcriptional Regulator SpxA1 Influences the Morphology and Virulence ofInfection and immunity · 2022Article
- Chirality of the cytoskeleton in the origins of cellular asymmetry.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- The cytoskeleton in cell-autonomous immunity: structural determinants of host defence.Nature reviews. Immunology · 2015Review
- Control of actin-based motility through localized actin binding.Physical biology · 2013Article
- Cell-cycle regulation of formin-mediated actin cable assembly.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Branching and capping determine the force-velocity relationships of branching actin networks.Physical biology · 2013Article
- Choosing orientation: influence of cargo geometry and ActA polarization on actin comet tails.Molecular biology of the cell · 2012Article
- Mesoscopic model of actin-based propulsion.PLoS computational biology · 2012Article
- Mitotic spindle assembly around RCC1-coated beads in Xenopus egg extracts.PLoS biology · 2011Article
- Observation and kinematic description of long actin tracks induced by spherical beads.Biophysical journal · 2010Article
- Force generation of curved actin gels characterized by combined AFM-epifluorescence measurements.Biophysical journal · 2010Article
- Force-velocity relation for actin-polymerization-driven motility from Brownian dynamics simulations.Biophysical journal · 2009Article
- In silico reconstitution of actin-based symmetry breaking and motility.PLoS biology · 2009Article
- Bio-mimetic surface engineering of plasmid-loaded nanoparticles for active intracellular trafficking by actin comet-tail motility.Biomaterials · 2009Article
- Active biological materials.Annual review of physical chemistry · 2009Review
- New proposed mechanism of actin-polymerization-driven motility.Biophysical journal · 2008Article
- Non-Gaussian curvature distribution of actin-propelled biomimetic colloid trajectories.European biophysics journal : EBJ · 2008Article
- Close packing of Listeria monocytogenes ActA, a natively unfolded protein, enhances F-actin assembly without dimerization.The Journal of biological chemistry · 2008Article
- Curvature and torsion in growing actin networks.Physical biology · 2008Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Using a biochemically complex cytoplasmic extract to reconstitute actin-based motility of Listeria monocytogenes and polystyrene beads coated with the bacterial protein ActA, we have systematically varied a series of biophysical parameters and examined their effects on initiation of motility, particle speed, speed variability, and path trajectory. Bead size had a profound effect on all aspects of motility, with increasing size causing slower, straighter movement and inhibiting symmetry-breaking. Speed also was reduced by extract dilution, by addition of methylcellulose, and paradoxically by addition of excess skeletal muscle actin, but it was enhanced by addition of nonmuscle (platelet) actin. Large, persistent individual variations in speed were observed for all conditions and their relative magnitude increased with extract dilution, indicating that persistent alterations in particle surface properties may be responsible for intrinsic speed variations. Trajectory curvature was increased for smaller beads and also for particles moving in the presence of methylcellulose or excess skeletal muscle actin. Symmetry breaking and movement initiation occurred by two distinct modes: either stochastic amplification of local variation for small beads in concentrated extracts, or gradual accumulation of strain in the actin gel for large beads in dilute extracts. Neither mode was sufficient to enable spherical particles to break symmetry in the cytoplasm of living cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.