ArticleJournal of clinical microbiology2004
Differential identification of Mycobacterium tuberculosis complex and nontuberculous mycobacteria by duplex PCR assay using the RNA polymerase gene (rpoB).
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 73 citations in OpenAlex.
- Miliary Tuberculosis due to Mycobacterium tuberculosis and Mycobacterium smegmatis associated with invasive aspergillosis in a renal transplant recipient.BMC nephrology · 2025Article
- Divergent proinflammatory immune responses associated with the differential susceptibility of cattle breeds to tuberculosis.Frontiers in immunology · 2023Article
- Modified genome comparison method: a new approach for identification of specific targets in molecular diagnostic tests using Mycobacterium tuberculosis complex as an example.BMC infectious diseases · 2018Article
- Performance of an in-house real-time polymerase chain reaction for identification of Mycobacterium tuberculosis isolates in laboratory routine diagnosis from a high burden setting.Memorias do Instituto Oswaldo Cruz · 2016Article
- Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria.Clinical microbiology reviews · 2016Review
- atpE gene as a new useful specific molecular target to quantify Mycobacterium in environmental samples.BMC microbiology · 2013Article
- Incidence of tuberculous and non-tuberculous mycobacteria, differentiated by multiplex PCR, in clinical specimens of a large general hospital.Clinics (Sao Paulo, Brazil) · 2013Article
- Molecular evidence of lateral gene transfer in rpoB gene of Mycobacterium yongonense strains via multilocus sequence analysis.PloS one · 2013Article
- Combined rpoB duplex PCR and hsp65 PCR restriction fragment length polymorphism with capillary electrophoresis as an effective algorithm for identification of mycobacterial species from clinical isolates.BMC microbiology · 2012Article
- The Use of High Performance Liquid Chromatography to Speciate and Characterize the Epidemiology of Mycobacteria.Laboratory medicine · 2011Article
- The role of speciation in positive Lowenstein-Jensen culture isolates from a high tuberculosis burden country.PloS one · 2011Article
- Study of Genetic Evolution in Mycobacterium tuberculosis Isolates from Patients with Active Pulmonary Tuberculosis in the Iran and Belarus.The open microbiology journal · 2011Article
- Development of a real-time qPCR method for detection and enumeration of Mycobacterium spp. in surface water.Applied and environmental microbiology · 2010Article
- Characterization of Molecular Evolution in Multi-Drug Resistant of Mycobacterium tuberculosis by rpoB Gene in Patient with Active Pulmonary Tuberculosis from Iranian Isolates.International journal of biomedical science : IJBS · 2009Article
- Molecular characterization of Capnocytophaga canimorsus and other canine Capnocytophaga spp. and assessment by PCR of their frequencies in dogs.Journal of clinical microbiology · 2009Article
- High-level rifampin resistance correlates with multiple mutations in the rpoB gene of pulmonary tuberculosis isolates from the Afghanistan border of Iran.Journal of clinical microbiology · 2009Article
- Species spectrum of nontuberculous mycobacteria isolated from clinical specimens in Kuwait.Current microbiology · 2008Article
- Differentiation of Mycobacterial species by hsp65 duplex PCR followed by duplex-PCR-based restriction analysis and direct sequencing.Journal of clinical microbiology · 2006Article
- Use of PCR and reverse line blot hybridization macroarray based on 16S-23S rRNA gene internal transcribed spacer sequences for rapid identification of 34 mycobacterium species.Journal of clinical microbiology · 2006Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel duplex PCR method that can amplify the 235- and 136-bp rpoB DNAs of Mycobacterium tuberculosis complex and nontuberculous mycobacteria (NTM), respectively, with two different sets of primers was used to differentially identify 44 reference strains and 379 clinical isolates of mycobacteria in a single-step assay. Showing 100% sensitivity and specificity, the duplex PCR method could clearly differentiate M. tuberculosis complex and NTM strains. In addition, restriction fragment length polymorphism analysis and direct sequencing of the amplicon of NTM could be used to supplement species identification.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.