ArticleJournal of clinical microbiology2004
Detection of orthopoxvirus DNA by real-time PCR and identification of variola virus DNA by melting analysis.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it, 89 citations in OpenAlex.
- Laboratory validation and clinical performance of a saliva-based test for monkeypox virus.Journal of medical virology · 2023Pooled it
- Engineered fluorescence for live diagnostics and quantification of poxvirus infection.The Journal of general virology · 2026Article
- A Case of Cowpox Presenting as Progressive Unilateral Facial Swelling and Fever Without Any Skin Lesions.Case reports in infectious diseases · 2026Article
- Monkeypox in Bulgaria: Significance of Various Clinical Samples, Clinical Manifestation, and Molecular Detection.Journal of clinical medicine · 2024Article
- Mapping the Landscape of Health Research Priorities for Effective Pandemic Preparedness in Human Mpox Virus Disease.Pathogens (Basel, Switzerland) · 2023Review
- Viral genetic clustering and transmission dynamics of the 2022 mpox outbreak in Portugal.Nature medicine · 2023Article
- Review
- Development of an amplicon-based sequencing approach in response to the global emergence of mpox.PLoS biology · 2023Article
- Development of an amplicon-based sequencing approach in response to the global emergence of human monkeypox virus.medRxiv : the preprint server for health sciences · 2023Article
- Review
- Ophthalmic Features and Implications of Poxviruses: Lessons from Clinical and Basic Research.Microorganisms · 2022Review
- Evaluation of 11 commercially available PCR kits for the detection of monkeypox virus DNA, Berlin, July to September 2022.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2022Article
- Current and Perspective Sensing Methods for Monkeypox Virus.Bioengineering (Basel, Switzerland) · 2022Review
- Article
- Discovery of novel DNA viruses in small mammals from Kenya.Virologica Sinica · 2022Article
- Ongoing monkeypox virus outbreak, Portugal, 29 April to 23 May 2022.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2022Article
- Smallpox in the Post-Eradication Era.Viruses · 2020Review
- Droplet digital PCR for rapid enumeration of viral genomes and particles from cells and animals infected with orthopoxviruses.Virology · 2017Article
- Article
- Classification of Cowpox Viruses into Several Distinct Clades and Identification of a Novel Lineage.Viruses · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although variola virus was eradicated by the World Health Organization vaccination program in the 1970s, the diagnosis of smallpox infection has attracted great interest in the context of a possible deliberate release of variola virus in bioterrorist attacks. Obviously, fast and reliable diagnostic tools are required to detect variola virus and to distinguish it from orthopoxviruses that have identical morphological characteristics, including vaccinia virus. The advent of real-time PCR for the clinical diagnosis of viral infections has facilitated the detection of minute amounts of viral nucleic acids in a fast, safe, and precise manner, including the option to quantify and to genotype the target reliably. In this study a complete set of four hybridization probe-based real-time PCR assays for the specific detection of orthopoxvirus DNA is presented. Melting analysis following PCR enables the identification of variola virus by the PCR product's characteristic melting temperature, permitting the discrimination of variola virus from other orthopoxviruses. In addition, an assay for the specific amplification of variola virus DNA is presented. All assays can be performed simultaneously in the same cycler, and results of a PCR run are obtained in less than 1 h. The application of more than one assay for the same organism significantly contributes to the diagnostic reliability, reducing the risk of false-negative results due to unknown sequence variations. In conclusion, the assays presented will improve the speed and reliability of orthopoxvirus diagnostics and variola virus identification.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.