ArticleJournal of clinical microbiology2004
Microarray-based identification of bacteria in clinical samples by solid-phase PCR amplification of 23S ribosomal DNA sequences.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 87 citations in OpenAlex.
- Harnessing Variabilities in Digital Melt Curves for Accurate Identification of Bacteria.Analytical chemistry · 2023Article
- Microfluidic-Based Nucleic Acid Amplification Systems in Microbiology.Micromachines · 2019Review
- A new microfluidic approach for the one-step capture, amplification and label-free quantification of bacteria from raw samples.Chemical science · 2017Article
- Microarray-based long oligonucleotides probe designed for Brucella Spp. detection and identification of antibiotic susceptibility pattern.Electronic physician · 2016Article
- LNA-modified isothermal oligonucleotide microarray for differentiating bacilli of similar origin.Journal of biosciences · 2014Article
- Immobilization techniques for microarray: challenges and applications.Sensors (Basel, Switzerland) · 2014Review
- Integrated Amplification Microarrays for Infectious Disease Diagnostics.Microarrays (Basel, Switzerland) · 2012Review
- Molecular detection, quantification, and diversity evaluation of microalgae.Marine biotechnology (New York, N.Y.) · 2012Review
- Application of phylogenetic microarrays to interrogation of human microbiota.FEMS microbiology ecology · 2012Review
- A universal method for the identification of bacteria based on general PCR primers.Indian journal of microbiology · 2011Article
- Development of a DNA microarray to detect antimicrobial resistance genes identified in the National Center for Biotechnology Information database.Microbial drug resistance (Larchmont, N.Y.) · 2010Article
- The role of DNA diffusion in solid phase polymerase chain reaction with gel-immobilized primers in planar and capillary microarray format.Biomicrofluidics · 2009Article
- Basic concepts of microarrays and potential applications in clinical microbiology.Clinical microbiology reviews · 2009Review
- High-throughput quantitative analysis of the human intestinal microbiota with a phylogenetic microarray.Applied and environmental microbiology · 2009Article
- [Microbiological diagnosis: Etiologic driven and syndrome driven].Antibiotiques (Paris, France : 1999) · 2009Article
- Microfluidic systems for pathogen sensing: a review.Sensors (Basel, Switzerland) · 2009Article
- Food microbial pathogen detection and analysis using DNA microarray technologies.Foodborne pathogens and disease · 2008Article
- Design and validation of a low density array (Nosochip) for the detection and identification of the main pathogenic bacteria and fungi responsible for nosocomial pneumonia.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2008Article
- Detection of Bacterial Pathogens in Cerebrospinal Fluid using Restriction Fragment Length Polymorphism.Medical journal, Armed Forces India · 2008Article
- Identification of upper respiratory tract pathogens using electrochemical detection on an oligonucleotide microarray.PloS one · 2007Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The rapid identification of the bacteria in clinical samples is important for patient management and antimicrobial therapy. We describe a DNA microarray-based PCR approach for the quick detection and identification of bacteria from cervical swab specimens from mares. This on-chip PCR method combines the amplification of a variable region of bacterial 23S ribosomal DNA and the simultaneous sequence-specific detection on a solid phase. The solid phase contains bacterial species-specific primers covalently bound to a glass support. During the solid-phase amplification reaction the polymerase elongates perfectly matched primers and incorporates biotin-labeled nucleotides. The reaction products are visualized by streptavidin-cyanine 5 staining, followed by fluorescence scanning. This procedure successfully identified from pure cultures 22 bacteria that are common causes of abortion and sterility in mares. Using the on-chip PCR method, we also tested 21 cervical swab specimens from mares for the presence of pathogenic bacteria and compared the results with those of conventional bacteriological culture methods. Our method correctly identified the bacteria in 12 cervical swab samples, 8 of which contained more than one bacterial species. Due to the higher sensitivity of the on-chip PCR, this method identified bacteria in five cervical swab samples which were not detected by the conventional identification procedure. Our results show that this method will have great potential to be incorporated into the routine microbiology laboratory.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.